CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011572
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform 
Protein Synonyms/Alias
 CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform 
Gene Name
 PPP3CA 
Gene Synonyms/Alias
 CALNA; CNA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32PSHRLTAKEVFDNDGubiquitination[1, 2]
142ALKILYPKTLFLLRGubiquitination[1]
219IRKLDRFKEPPAYGPubiquitination[2]
323NNKAAVLKYENNVMNubiquitination[2]
424SESVLTLKGLTPTGMubiquitination[2]
441SGVLSGGKQTLQSAIubiquitination[1, 2, 3]
456KGFSPQHKITSFEEAubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. 
Sequence Annotation
 REGION 1 301 Catalytic.
 REGION 247 253 Calcineurin B binding-site 1 (Potential).
 REGION 296 301 Calcineurin B binding-site 2 (Potential).
 REGION 392 414 Calmodulin-binding (Potential).
 REGION 465 487 Inhibitory domain.
 ACT_SITE 151 151 Proton donor (By similarity).
 METAL 90 90 Iron.
 METAL 92 92 Iron.
 METAL 118 118 Iron.
 METAL 118 118 Zinc.
 METAL 150 150 Zinc.
 METAL 199 199 Zinc.
 METAL 281 281 Zinc.
 MOD_RES 224 224 Nitrated tyrosine (By similarity).
 MOD_RES 469 469 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Calmodulin-binding; Complete proteome; Hydrolase; Iron; Metal-binding; Nitration; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 521 AA 
Protein Sequence
MSEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL MKEGRLEESV 60
ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK LFEVGGSPAN TRYLFLGDYV 120
DRGYFSIECV LYLWALKILY PKTLFLLRGN HECRHLTEYF TFKQECKIKY SERVYDACMD 180
AFDCLPLAAL MNQQFLCVHG GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG 240
NEKTQEHFTH NTVRGCSYFY SYPAVCEFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP 300
SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF TWSLPFVGEK 360
VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI RAIGKMARVF SVLREESESV 420
LTLKGLTPTG MLPSGVLSGG KQTLQSAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM 480
PSDANLNSIN KALTSETNGT DSNGSNSSNI Q 511 
Gene Ontology
 GO:0005955; C:calcineurin complex; NAS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0030018; C:Z disc; IEA:Compara.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0005516; F:calmodulin binding; IDA:UniProtKB.
 GO:0033192; F:calmodulin-dependent protein phosphatase activity; IDA:UniProtKB.
 GO:0008144; F:drug binding; IDA:UniProtKB.
 GO:0019899; F:enzyme binding; IDA:UniProtKB.
 GO:0033173; P:calcineurin-NFAT signaling cascade; IEA:Compara.
 GO:0006816; P:calcium ion transport; IEA:Compara.
 GO:0035690; P:cellular response to drug; IDA:UniProtKB.
 GO:0071333; P:cellular response to glucose stimulus; IEA:Compara.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0046676; P:negative regulation of insulin secretion; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006470; P:protein dephosphorylation; IDA:UniProtKB.
 GO:0006606; P:protein import into nucleus; IEA:Compara.
 GO:0060079; P:regulation of excitatory postsynaptic membrane potential; IEA:Compara.
 GO:0050804; P:regulation of synaptic transmission; IEA:Compara.
 GO:0001975; P:response to amphetamine; IEA:Compara.
 GO:0051592; P:response to calcium ion; IDA:UniProtKB.
 GO:0006950; P:response to stress; IEA:Compara.
 GO:0048741; P:skeletal muscle fiber development; IEA:Compara.
 GO:0042110; P:T cell activation; TAS:UniProtKB.
 GO:0014883; P:transition between fast and slow fiber; IEA:Compara. 
Interpro
 IPR004843; Metallo_PEstase_dom.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 
Pfam
 PF00149; Metallophos 
SMART
 SM00156; PP2Ac 
PROSITE
 PS00125; SER_THR_PHOSPHATASE 
PRINTS
 PR00114; STPHPHTASE.