CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008566
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup98-Nup96 
Protein Synonyms/Alias
 Nuclear pore complex protein Nup98; 98 kDa nucleoporin; Nucleoporin Nup98; Nup98; Nuclear pore complex protein Nup96; 96 kDa nucleoporin; Nucleoporin Nup96; Nup96 
Gene Name
 NUP98 
Gene Synonyms/Alias
 ADAR2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
193HQCITAMKEYESKSLubiquitination[1]
198AMKEYESKSLEELRLubiquitination[1]
534KKKEERLKPTNPAAQubiquitination[1]
542PTNPAAQKALTTPTHubiquitination[1]
551LTTPTHYKLTPRPATubiquitination[1, 2]
563PATRVRPKALQTTGTubiquitination[1]
572LQTTGTAKSHLFDGLubiquitination[1, 3]
603SIKKLVLKNLNNSNLacetylation[4]
603SIKKLVLKNLNNSNLubiquitination[1, 2, 3, 5]
639ERFSFLSKPVDENHQubiquitination[1, 3]
665FYTNPIAKPIPQTPEubiquitination[3]
752PSMDDLAKITNEKGEubiquitination[3]
757LAKITNEKGECIVSDubiquitination[1]
818VGEGLNRKAEVTLDGubiquitination[1, 3]
831DGVWPTDKTSRCLIKubiquitination[1]
838KTSRCLIKSPDRLADubiquitination[1]
925LQMALNGKPAPPPQSubiquitination[3]
1019RFSRLPSKADTSQEIubiquitination[1]
1040ISASHSSKTRSLVGGubiquitination[1]
1200PFKVHLEKLSLRQRKubiquitination[1]
1213RKPDEDMKLYQTPLEubiquitination[1]
1222YQTPLELKLKHSTVHubiquitination[1, 3]
1224TPLELKLKHSTVHVDubiquitination[1]
1341VFSYLTGKRISEACSubiquitination[1, 2, 3, 5, 6]
1405IFALLAGKPVWQLSEubiquitination[1]
1413PVWQLSEKKQINVCSubiquitination[1]
1414VWQLSEKKQINVCSQubiquitination[1]
1425VCSQLDWKRSLAIHLubiquitination[1]
1497DVCFHLLKLYSDRHYubiquitination[1]
1601ETPESWAKETFLTQKubiquitination[1]
1608KETFLTQKLRVPAKWubiquitination[1, 5]
1614QKLRVPAKWIHEAKAubiquitination[1]
1620AKWIHEAKAVRAHMEubiquitination[1]
1630RAHMESDKHLEALCLubiquitination[1]
1639LEALCLFKAEHWNRCubiquitination[1]
1648EHWNRCHKLIIRHLAubiquitination[1]
1722DLEQLHIKVTSLCSRubiquitination[1]
1739QIQCYSAKDRLAQSDubiquitination[1]
1749LAQSDMAKRVANLLRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. 
Sequence Annotation
 DOMAIN 738 880 Peptidase S59.
 REGION 1 156 FG repeats 1.
 REGION 157 213 GLEBS; interaction with RAE1.
 REGION 214 480 FG repeats 2.
 MOD_RES 524 524 Phosphoserine.
 MOD_RES 603 603 N6-acetyllysine.
 MOD_RES 608 608 Phosphoserine.
 MOD_RES 612 612 Phosphoserine.
 MOD_RES 618 618 Phosphoserine.
 MOD_RES 623 623 Phosphoserine.
 MOD_RES 625 625 Phosphoserine.
 MOD_RES 670 670 Phosphothreonine.
 MOD_RES 673 673 Phosphoserine.
 MOD_RES 681 681 Phosphoserine.
 MOD_RES 683 683 Phosphoserine.
 MOD_RES 839 839 Phosphoserine.
 MOD_RES 888 888 Phosphoserine.
 MOD_RES 934 934 Phosphoserine.
 MOD_RES 1000 1000 Phosphothreonine.
 MOD_RES 1023 1023 Phosphoserine.
 MOD_RES 1028 1028 Phosphoserine.
 MOD_RES 1043 1043 Phosphoserine.
 MOD_RES 1060 1060 Phosphoserine.
 MOD_RES 1070 1070 Phosphothreonine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Autocatalytic cleavage; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; Host-virus interaction; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1817 AA 
Protein Sequence
MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG 60
GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF 120
GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST 180
NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL 240
FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF 300
SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV 360
GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNTSGNSIF 420
GSKPAPGTLG TGLGAGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTTTATLGFG 480
APQAPVALTD PNASAAQQAV LQQHINSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA 540
QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL 600
VLKNLNNSNL FSPVNRDSEN LASPSEYPEN GERFSFLSKP VDENHQQDGD EDSLVSHFYT 660
NPIAKPIPQT PESAGNKHSN SNSVDDTIVA LNMRAALRNG LEGSSEETSF HDESLQDDRE 720
EIENNSYHMH PAGIILTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD 780
VNLTNLNLDD IVHIRRKEVV VYLDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP 840
DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPSKTS 900
TKKLKTAPLP PASQTTPLQM ALNGKPAPPP QSQSPEVEQL GRVVELDSDM VDITQEPVLD 960
TMLEESMPED QEPVSASTHI ASSLGINPHV LQIMKASLLT DEEDVDMALD QRFSRLPSKA 1020
DTSQEICSPR LPISASHSSK TRSLVGGLLQ SKFTSGAFLS PSVSVQECRT PRAASLMNIP 1080
STSSWSVPPP LTSVFTMPSP APEVPLKTVG TRRQLGLVPR EKSVTYGKGK LLMDMALFMG 1140
RSFRVGWGPN WTLANSGEQL NGSHELENHQ IADSMEFGFL PNPVAVKPLT ESPFKVHLEK 1200
LSLRQRKPDE DMKLYQTPLE LKLKHSTVHV DELCPLIVPN LGVAVIHDYA DWVKEASGDL 1260
PEAQIVKHWS LTWTLCEALW GHLKELDSQL NEPREYIQIL ERRRAFSRWL SCTATPQIEE 1320
EVSLTQKNSP VEAVFSYLTG KRISEACSLA QQSGDHRLAL LLSQFVGSQS VRELLTMQLV 1380
DWHQLQADSF IQDERLRIFA LLAGKPVWQL SEKKQINVCS QLDWKRSLAI HLWYLLPPTA 1440
SISRALSMYE EAFQNTSDSD RYACSPLPSY LEGSGCVIAE EQNSQTPLRD VCFHLLKLYS 1500
DRHYDLNQLL EPRSITADPL DYRLSWHLWE VLRALNYTHL SAQCEGVLQA SYAGQLESEG 1560
LWEWAIFVLL HIDNSGIREK AVRELLTRHC QLLETPESWA KETFLTQKLR VPAKWIHEAK 1620
AVRAHMESDK HLEALCLFKA EHWNRCHKLI IRHLASDAII NENYDYLKGF LEDLAPPERS 1680
SLIQDWETSG LVYLDYIRVI EMLRHIQQVD CSGNDLEQLH IKVTSLCSRI EQIQCYSAKD 1740
RLAQSDMAKR VANLLRVVLS LHHPPDRTSD STPDPQRVPL RLLAPHIGRL PMPEDYAMDE 1800
LRSLTQSYLR ELAVGSL 1817 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0042405; C:nuclear inclusion body; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IDA:UniProtKB.
 GO:0034399; C:nuclear periphery; IDA:UniProtKB.
 GO:0044615; C:nuclear pore nuclear basket; IDA:UniProtKB.
 GO:0031080; C:nuclear pore outer ring; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:ProtInc.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0017056; F:structural constituent of nuclear pore; IMP:UniProtKB.
 GO:0005215; F:transporter activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0006260; P:DNA replication; IMP:UniProtKB.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0051292; P:nuclear pore complex assembly; IMP:UniProtKB.
 GO:0000059; P:protein import into nucleus, docking; NAS:UniProtKB.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR021967; Nup96.
 IPR007230; Peptidase_S59. 
Pfam
 PF04096; Nucleoporin2
 PF12110; Nup96 
SMART
  
PROSITE
 PS51434; NUP_C 
PRINTS