CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014470
UniProt Accession
Genbank Protein ID
 U47110 
Genbank Nucleotide ID
Protein Name
 Peripheral plasma membrane protein CASK 
Protein Synonyms/Alias
 Calcium/calmodulin-dependent serine protein kinase 
Gene Name
 Cask 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
41TGQQFAVKIVDVAKFacetylation[1]
41TGQQFAVKIVDVAKFubiquitination[2]
277ALNHPWLKERDRYAYacetylation[1]
653QGKLENSKNGTAGLIubiquitination[2]
679CIAMEKTKQEQQASCsumoylation[3]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [3] SUMOylation of the MAGUK protein CASK regulates dendritic spinogenesis.
 Chao HW, Hong CJ, Huang TN, Lin YL, Hsueh YP.
 J Cell Biol. 2008 Jul 14;182(1):141-55. [PMID: 18606847] 
Functional Description
 Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. 
Sequence Annotation
 DOMAIN 12 276 Protein kinase.
 DOMAIN 343 398 L27 1.
 DOMAIN 402 455 L27 2.
 DOMAIN 489 564 PDZ.
 DOMAIN 600 670 SH3.
 DOMAIN 722 894 Guanylate kinase-like.
 NP_BIND 18 26 ATP (By similarity).
 REGION 305 315 Calmodulin-binding.
 ACT_SITE 141 141 By similarity.
 BINDING 41 41 ATP (By similarity).
 MOD_RES 151 151 Phosphoserine; by autocatalysis (By
 MOD_RES 155 155 Phosphoserine; by autocatalysis (By
 MOD_RES 571 571 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; ATP-binding; Calmodulin-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 909 AA 
Protein Sequence
MADDDVLFED VYELCEVIGK GPFSVVRRCI NRETGQQFAV KIVDVAKFTS SPGLSTEDLK 60
REASICHMLK HPHIVELLET YSSDGMLYMV FEFMDGADLC FEIVKRADAG FVYSEAVASH 120
YMRQILEALR YCHDNNIIHR DVKPHCVLLA SKENSAPVKL GGFGVAIQLG ESGLVAGGRV 180
GTPHFMAPEV VKREPYGKPV DVWGCGVILF ILLSGCLPFY GTKERLFEGI IKGKYKMNPR 240
QWSHISESAK DLVRRMLMLD PAERITVYEA LNHPWLKERD RYAYKIHLPE TVEQLRKFNA 300
RRKLKGAVLA AVSSHKFNSF YGDPPEELPD FSEDPTSSGL LAAERAVSQV LDSLEEIHAL 360
TDCSEKDLDF LHSVFQDQHL HTLLDLYDKI NTKSSPQIRN PPSDAVQRAK EVLEEISCYP 420
ENNDAKELKR ILTQPHFMAL LQTHHVVAHE VYSDEALRVT PPPTSPYLNG DSPESANGDM 480
DMENVTRVRL VQFQKNTDEP MGITLKMNEL NHCIVARIMH GGMIHRQGTL HVGDEIREIN 540
GISVANQTVE QLQKMLREMR GSITFKIVPS YRTQSSSCER DSPSTSRQSP ANGHSSTNNS 600
VSIYVRAQFE YDPAKDDLIP CKEAGIRFRV GDIIQIISKD DHNWWQGKLE NSKNGTAGLI 660
PSPELQEWRV ACIAMEKTKQ EQQASCTWFG KKKKQYKDKY LAKHNADLVT YEEVVKLPAF 720
KRKTLVLLGA HGVGRRHIKN TLITKHPERF AYPIPHTTRP PKKDEENGKN YYFVSHDQMM 780
QDISNNEYLE YGSHEDAMYG TKLETIRKIH EQGLIAILDV EPQALKVLRT AEFPPFVVFI 840
AAPTITPGLN EDESLQRLQK ESDVLQRTYA HYFDLTIINN EIDETIRHLE EAVELVCTAP 900
QWVPVSWVY 909 
Gene Ontology
 GO:0060170; C:cilium membrane; IDA:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; IDA:RGD.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0042734; C:presynaptic membrane; IDA:BHF-UCL.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005516; F:calmodulin binding; TAS:RGD.
 GO:0004683; F:calmodulin-dependent protein kinase activity; TAS:RGD.
 GO:0008022; F:protein C-terminus binding; IMP:RGD.
 GO:0003713; F:transcription coactivator activity; TAS:RGD.
 GO:0006886; P:intracellular protein transport; IMP:RGD.
 GO:0006461; P:protein complex assembly; TAS:RGD. 
Interpro
 IPR008144; Guanylate_kin.
 IPR008145; Guanylate_kin/L-typ_Ca_channel.
 IPR020590; Guanylate_kinase_CS.
 IPR011009; Kinase-like_dom.
 IPR004172; L27.
 IPR014775; L27_C.
 IPR027417; P-loop_NTPase.
 IPR001478; PDZ.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00625; Guanylate_kin
 PF02828; L27
 PF00595; PDZ
 PF00069; Pkinase
 PF07653; SH3_2 
SMART
 SM00072; GuKc
 SM00569; L27
 SM00228; PDZ
 SM00220; S_TKc
 SM00326; SH3 
PROSITE
 PS00856; GUANYLATE_KINASE_1
 PS50052; GUANYLATE_KINASE_2
 PS51022; L27
 PS50106; PDZ
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50002; SH3 
PRINTS