CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010095
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Proline--tRNA ligase 
Protein Synonyms/Alias
 Prolyl-tRNA synthetase; ProRS 
Gene Name
 proS 
Gene Synonyms/Alias
 MPN_402; MP436 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
310VKLYLFDKKGDTKTVacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) (By similarity). 
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 483 AA 
Protein Sequence
MANKDQNLTL WYDQLLSKAQ LVSYGDVKGT NCFLPNSWNL WLQIQRLYNN ATALIKLKDK 60
VILKQFIPIE PLPYTVEQVQ LPTLSFYSEY QKEKRHVEGF NPELFLIEQI GTKKLHDPLV 120
LRPTSEIAFC NLWKKQSFSY QNLPVIYNQW TCVFRAEKNT RPFLRNSEFY WQETHGLFSD 180
GVNSESAAIA FWKLYQDIIV NQLCIPAFVG LKSPNERFAG AQNTWTVESI MPDGQALQCA 240
TSHDLGQTFT KPFGLTFQNQ ANQQAIPYSF SCGISTRILG ALLLTHSDDF GLVLPWKVAP 300
IQVKLYLFDK KGDTKTVELA QKVQTLLEQL AIRFQFIKVE NQLGKQLGQG EVNGIPFQLI 360
VDNPQTVNIF NRLTRVKTAY SFEQLASRFV ELVQQYHQAM YDKAKAVVQQ KVVQATTLKQ 420
IEQAFNDKKA VLCAVRLTDT LEQQLKERYQ VTVRCCLEQL QKPQICPFSG ESAQDYVLIA 480
RAY 483 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004827; F:proline-tRNA ligase activity; IEA:EC.
 GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR002316; Pro-tRNA-ligase_IIa.
 IPR004499; Pro-tRNA-ligase_IIa_arc-type.
 IPR016061; Pro-tRNA_ligase_II_C.
 IPR017449; Pro-tRNA_synth_II. 
Pfam
 PF03129; HGTP_anticodon
 PF09180; ProRS-C_1
 PF00587; tRNA-synt_2b 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01046; TRNASYNTHPRO.