CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001727
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome b5 
Protein Synonyms/Alias
 Microsomal cytochrome b5 type A; MCB5 
Gene Name
 CYB5A 
Gene Synonyms/Alias
 CYB5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10EQSDEAVKYYTLEEIubiquitination[1, 2]
19YTLEEIQKHNHSKSTacetylation[3, 4]
39HKVYDLTKFLEEHPGacetylation[5]
39HKVYDLTKFLEEHPGubiquitination[1, 2, 6, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. 
Sequence Annotation
 DOMAIN 9 85 Cytochrome b5 heme-binding.
 METAL 44 44 Iron (heme axial ligand).
 METAL 68 68 Iron (heme axial ligand).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 19 19 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Electron transport; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 134 AA 
Protein Sequence
MAEQSDEAVK YYTLEEIQKH NHSKSTWLIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT 60
ENFEDVGHST DAREMSKTFI IGELHPDDRP KLNKPPETLI TTIDSSSSWW TNWVIPAISA 120
VAVALMYRLY MAED 134 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; TAS:Reactome.
 GO:0004033; F:aldo-keto reductase (NADP) activity; TAS:Reactome.
 GO:0004129; F:cytochrome-c oxidase activity; TAS:ProtInc.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0019852; P:L-ascorbic acid metabolic process; TAS:Reactome. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR018506; Cyt_B5_heme-BS. 
Pfam
 PF00173; Cyt-b5 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS
 PR00363; CYTOCHROMEB5.