CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011882
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phospholipid-transporting ATPase DNF2 
Protein Synonyms/Alias
 Flippase DNF2 
Gene Name
 DNF2 
Gene Synonyms/Alias
 YDR093W; YD8557.01 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
99DDDELNGKKTEIHEHubiquitination[1]
938NDATLLEKTALHLEEubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids. Required for protein transport from Golgi to vacuoles. 
Sequence Annotation
 ACT_SITE 712 712 4-aspartylphosphate intermediate
 MOD_RES 70 70 Phosphothreonine.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 389 389 Phosphoserine.
 MOD_RES 392 392 Phosphoserine.
 MOD_RES 396 396 Phosphoserine.
 MOD_RES 403 403 Phosphoserine.
 MOD_RES 406 406 Phosphotyrosine.
 MOD_RES 782 782 Phosphothreonine.
 MOD_RES 1542 1542 Phosphoserine.
 MOD_RES 1592 1592 Phosphoserine.  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Hydrolase; Lipid transport; Magnesium; Membrane; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1612 AA 
Protein Sequence
MSSPSKPTSP FVDDIEHESG SASNGLSSMS PFDDSFQFEK PSSAHGNIEV AKTGGSVLKR 60
QSKPMKDIST PDLSKVTFDG IDDYSNDNDI NDDDELNGKK TEIHEHENEV DDDLHSFQAT 120
PMPNTGGFED VELDNNEGSN NDSQADHKLK RVRFGTRRNK SGRIDINRSK TLKWAKKNFH 180
NAIDEFSTKE DSLENSALQN RSDELRTVYY NLPLPEDMLD EDGLPLAVYP RNKIRTTKYT 240
PLTFFPKNIL FQFHNFANIY FLILLILGAF QIFGVTNPGF ASVPLIVIVI ITAIKDGIED 300
SRRTVLDLEV NNTRTHILSG VKNENVAVDN VSLWRRFKKA NTRALIKIFE YFSENLTAAG 360
REKKLQKKRE ELRRKRNSRS FGPRGSLDSI GSYRMSADFG RPSLDYENLN QTMSQANRYN 420
DGENLVDRTL QPNPECRFAK DYWKNVKVGD IVRVHNNDEI PADMILLSTS DVDGACYVET 480
KNLDGETNLK VRQSLKCSKI IKSSRDITRT KFWVESEGPH ANLYSYQGNF KWQDTQNGNI 540
RNEPVNINNL LLRGCTLRNT KWAMGMVIFT GDDTKIMINA GVTPTKKSRI SRELNFSVIL 600
NFVLLFILCF TAGIVNGVYY KQKPRSRDYF EFGTIGGSAS TNGFVSFWVA VILYQSLVPI 660
SLYISVEIIK TAQAIFIYTD VLLYNAKLDY PCTPKSWNIS DDLGQIEYIF SDKTGTLTQN 720
VMEFKKCTIN GVSYGRAYTE ALAGLRKRQG VDVESEGRRE KEEIAKDRET MIDELRSMSD 780
NTQFCPEDLT FVSKEIVEDL KGSSGDHQQK CCEHFLLALA LCHSVLVEPN KDDPKKLDIK 840
AQSPDESALV STARQLGYSF VGSSKSGLIV EIQGVQKEFQ VLNVLEFNSS RKRMSCIIKI 900
PGSTPKDEPK ALLICKGADS VIYSRLDRTQ NDATLLEKTA LHLEEYATEG LRTLCLAQRE 960
LTWSEYERWV KTYDVAAASV TNREEELDKV TDVIERELIL LGGTAIEDRL QDGVPDSIAL 1020
LAEAGIKLWV LTGDKVETAI NIGFSCNVLN NDMELLVVKA SGEDVEEFGS DPIQVVNNLV 1080
TKYLREKFGM SGSEEELKEA KREHGLPQGN FAVIIDGDAL KVALNGEEMR RKFLLLCKNC 1140
KAVLCCRVSP AQKAAVVKLV KKTLDVMTLA IGDGSNDVAM IQSADVGVGI AGEEGRQAVM 1200
CSDYAIGQFR YVTRLVLVHG KWCYKRLAEM IPQFFYKNVI FTLSLFWYGI YNNFDGSYLF 1260
EYTYLTFYNL AFTSVPVILL AVLDQDVSDT VSMLVPQLYR VGILRKEWNQ TKFLWYMLDG 1320
VYQSVICFFF PYLAYHKNMV VTENGLGLDH RYFVGVFVTA IAVTSCNFYV FMEQYRWDWF 1380
CGLFICLSLA VFYGWTGIWT SSSSSNEFYK GAARVFAQPA YWAVLFVGVL FCLLPRFTID 1440
CIRKIFYPKD IEIVREMWLR GDFDLYPQGY DPTDPSRPRI NEIRPLTDFK EPISLDTHFD 1500
GVSHSQETIV TEEIPMSILN GEQGSRKGYR VSTTLERRDQ LSPVTTTNNL PRRSMASARG 1560
NKLRTSLDRT REEMLANHQL DTRYSVERAR ASLDLPGINH AETLLSQRSR DR 1612 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019829; F:cation-transporting ATPase activity; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004012; F:phospholipid-translocating ATPase activity; IMP:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0007163; P:establishment or maintenance of cell polarity; IGI:SGD.
 GO:0006886; P:intracellular protein transport; IGI:SGD.
 GO:0045332; P:phospholipid translocation; IMP:SGD. 
Interpro
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR006539; ATPase_P-typ_Plipid-transp.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom.
 IPR026871; PLip_transp_ATPase_fungal. 
Pfam
 PF00122; E1-E2_ATPase 
SMART
  
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS