CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001245
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein phosphatase 1B 
Protein Synonyms/Alias
 Protein phosphatase 2C isoform beta; PP2C-beta 
Gene Name
 PPM1B 
Gene Synonyms/Alias
 PP2CB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
112GIRTGFLKIDEYMRNubiquitination[1]
326RVEEIMEKSGEEGMPubiquitination[1, 2]
391EESGSQGKLVEALRQubiquitination[1, 2, 3, 4, 5]
422LTSYRLAKVEGEESPubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. 
Sequence Annotation
 METAL 60 60 Manganese 1 (By similarity).
 METAL 60 60 Manganese 2 (By similarity).
 METAL 61 61 Manganese 1; via carbonyl oxygen (By
 METAL 243 243 Manganese 2 (By similarity).
 METAL 286 286 Manganese 2 (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 479 AA 
Protein Sequence
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL EDWSFFAVYD 60
GHAGSRVANY CSTHLLEHIT TNEDFRAAGK SGSALELSVE NVKNGIRTGF LKIDEYMRNF 120
SDLRNGMDRS GSTAVGVMIS PKHIYFINCG DSRAVLYRNG QVCFSTQDHK PCNPREKERI 180
QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL 240
ACDGIWDVMS NEELCEYVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK 300
VSDEAVKKDS ELDKHLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 360
NVIEAVYSRL NPHRESDGAS DEAEESGSQG KLVEALRQMR INHRGNYRQL LEEMLTSYRL 420
AKVEGEESPA EPAATATSSN SDAGNPVTMQ ESHTESESGL AELDSSNEDA GTKMSGEKI 479 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0016020; C:membrane; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0006499; P:N-terminal protein myristoylation; ISS:UniProtKB.
 GO:0050687; P:negative regulation of defense response to virus; IMP:UniProtKB.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0032688; P:negative regulation of interferon-beta production; IMP:UniProtKB.
 GO:0042347; P:negative regulation of NF-kappaB import into nucleus; IMP:UniProtKB.
 GO:0035970; P:peptidyl-threonine dephosphorylation; IDA:UniProtKB. 
Interpro
 IPR001932; PP2C-like.
 IPR012911; PP2C_C.
 IPR000222; PP2C_Mn2_Asp60_BS.
 IPR015655; Protein_Pase_2C. 
Pfam
 PF00481; PP2C
 PF07830; PP2C_C 
SMART
 SM00331; PP2C_SIG
 SM00332; PP2Cc 
PROSITE
 PS01032; PP2C 
PRINTS