CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022572
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA-binding protein 27 
Protein Synonyms/Alias
 RNA-binding motif protein 27 
Gene Name
 RBM27 
Gene Synonyms/Alias
 KIAA1311 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
653LTNEEARKAISSTEAubiquitination[1, 2]
734GIQKMMSKPQTSGAYmethylation[3]
1003STANQGPKFKDRRLQubiquitination[2, 4]
Reference
 [1] Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
 Tan F, Lu L, Cai Y, Wang J, Xie Y, Wang L, Gong Y, Xu BE, Wu J, Luo Y, Qiang B, Yuan J, Sun X, Peng X.
 Proteomics. 2008 Jul;8(14):2885-96. [PMID: 18655026]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
 DOMAIN 600 674 RRM.
 ZN_FING 273 301 C3H1-type.
 MOD_RES 120 120 Phosphoserine.
 MOD_RES 447 447 Phosphothreonine.
 MOD_RES 1020 1020 Phosphoserine.
 CROSSLNK 653 653 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1060 AA 
Protein Sequence
MLIEDVDALK SWLAKLLEPI CDADPSALAN YVVALVKKDK PEKELKAFCA DQLDVFLQKE 60
TSGFVDKLFE SLYTKNYLPL LEPVKPEPKP LVQEKEEIKE EVFQEPAEEE RDGRKKKYPS 120
PQKTRSESSE RRTREKKRED GKWRDYDRYY ERNELYREKY DWRRGRSKSR SKSRGLSRSR 180
SRSRGRSKDR DPNRNVEHRE RSKFKSERND LESSYVPVSA PPPNSSEQYS SGAQSIPSTV 240
TVIAPAHHSE NTTESWSNYY NNHSSSNSFG RNLPPKRRCR DYDERGFCVL GDLCQFDHGN 300
DPLVVDEVAL PSMIPFPPPP PGLPPPPPPG MLMPPMPGPG PGPGPGPGPG PGPGPGPGHS 360
MRLPVPQGHG QPPPSVVLPI PRPPITQSSL INSRDQPGTS AVPNLASVGT RLPPPLPQNL 420
LYTVSERQPM YSREHGAAAS ERLQLGTPPP LLAARLVPPR NLMGSSIGYH TSVSSPTPLV 480
PDTYEPDGYN PEAPSITSSG RSQYRQFFSR TQTQRPNLIG LTSGDMDVNP RAANIVIQTE 540
PPVPVSINSN ITRVVLEPDS RKRAMSGLEG PLTKKPWLGK QGNNNQNKPG FLRKNQYTNT 600
KLEVKKIPQE LNNITKLNEH FSKFGTIVNI QVAFKGDPEA ALIQYLTNEE ARKAISSTEA 660
VLNNRFIRVL WHRENNEQPT LQSSAQLLLQ QQQTLSHLSQ QHHHLPQHLH QQQVLVAQSA 720
PSTVHGGIQK MMSKPQTSGA YVLNKVPVKH RLGHAGGNQS DASHLLNQSG GAGEDCQIFS 780
TPGHPKMIYS SSNLKTPSKL CSGSKSHDVQ EVLKKKQEAM KLQQDMRKKR QEVLEKQIEC 840
QKMLISKLEK NKNMKPEERA NIMKTLKELG EKISQLKDEL KTSSAVSTPS KVKTKTEAQK 900
ELLDTELDLH KRLSSGEDTT ELRKKLSQLQ VEAARLGILP VGRGKTMSSQ GRGRGRGRGG 960
RGRGSLNHMV VDHRPKALTV GGFIEEEKED LLQHFSTANQ GPKFKDRRLQ ISWHKPKVPS 1020
ISTETEEEEV KEEETETSDL FLPDDDDEDE DEYESRSWRR 1060 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:InterPro. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR002483; PWI_dom.
 IPR000504; RRM_dom.
 IPR000571; Znf_CCCH. 
Pfam
 PF01480; PWI
 PF00642; zf-CCCH 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM
 PS50103; ZF_C3H1 
PRINTS