CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015482
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ceramide synthase 6 
Protein Synonyms/Alias
 CerS5; LAG1 longevity assurance homolog 6 
Gene Name
 CERS6 
Gene Synonyms/Alias
 LASS6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92KVFTAITKHPDEKRLubiquitination[1, 2]
97ITKHPDEKRLEGLSKubiquitination[1]
104KRLEGLSKQLDWDVRubiquitination[1, 2, 3, 4, 5]
125RQRRNQEKPSTLTRFubiquitination[1, 2]
155YGVRFLKKTPWLWNTubiquitination[1]
255AKMANYAKFQKMCDLubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May be involved in sphingolipid synthesis or its regulation (By similarity). 
Sequence Annotation
 DOMAIN 130 331 TLC.
 DNA_BIND 66 127 Homeobox.
 CARBOHYD 18 18 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Endoplasmic reticulum; Glycoprotein; Homeobox; Lipid biosynthesis; Lipid metabolism; Membrane; Nucleus; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 384 AA 
Protein Sequence
MAGILAWFWN ERFWLPHNVT WADLKNTEEA TFPQAEDLYL AFPLAFCIFM VRLIFERFVA 60
KPCAIALNIQ ANGPQIAPPN AILEKVFTAI TKHPDEKRLE GLSKQLDWDV RSIQRWFRQR 120
RNQEKPSTLT RFCESMWRFS FYLYVFTYGV RFLKKTPWLW NTRHCWYNYP YQPLTTDLHY 180
YYILELSFYW SLMFSQFTDI KRKDFGIMFL HHLVSIFLIT FSYVNNMARV GTLVLCLHDS 240
ADALLEAAKM ANYAKFQKMC DLLFVMFAVV FITTRLGIFP LWVLNTTLFE SWEIVGPYPS 300
WWVFNLLLLL VQGLNCFWSY LIVKIACKAV SRGKVSKDDR SDIESSSDEE DSEPPGKNPH 360
TATTTNGTSG TNGYLLTGSC SMDD 384 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0050291; F:sphingosine N-acyltransferase activity; IEA:Compara.
 GO:0046513; P:ceramide biosynthetic process; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0030148; P:sphingolipid biosynthetic process; TAS:Reactome. 
Interpro
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR016439; Longevity_assurance_LAG1_LAC1.
 IPR006634; TLC-dom. 
Pfam
 PF00046; Homeobox
 PF03798; TRAM_LAG1_CLN8 
SMART
 SM00389; HOX
 SM00724; TLC 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS50922; TLC 
PRINTS