CPLM-000120

Genbank Nucleotide ID

Serine/threonine-protein phosphatase 6 catalytic subunit

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
8	MAPLDLDKYVEIARL	ubiquitination	[1, 2, 3, 4]
17	VEIARLCKYLPENDL	ubiquitination	[3, 4, 5]
25	YLPENDLKRLCDYVC	ubiquitination	[3]
132	FYDECQTKYGNANAW	ubiquitination	[3, 5, 6, 7]
188	RNQEIPHKGAFCDLV	ubiquitination	[3]
219	AGWLFGAKVTNEFVH	ubiquitination	[3]
231	FVHINNLKLICRAHQ	ubiquitination	[1, 3]
283	DVNTREPKLFRAVPD	ubiquitination	[3]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]

Functional Description

Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.

ACT_SITE 114 114 Proton donor (By similarity).
METAL 53 53 Iron (By similarity).
METAL 55 55 Iron (By similarity).
METAL 81 81 Iron (By similarity).
METAL 81 81 Manganese (By similarity).
METAL 113 113 Manganese (By similarity).
METAL 163 163 Manganese (By similarity).
METAL 237 237 Manganese (By similarity).

Alternative splicing; Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Iron; Manganese; Metal-binding; Protein phosphatase; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:MGI.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0004722; F:protein serine/threonine phosphatase activity; IDA:MGI.
GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:ProtInc.
GO:0006470; P:protein dephosphorylation; IDA:MGI.

IPR004843; Metallo_PEstase_dom.
IPR006186; Ser/Thr-sp_prot-phosphatase.

PF00149; Metallophos

PS00125; SER_THR_PHOSPHATASE

PR00114; STPHPHTASE.