CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012700
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cleavage and polyadenylation specificity factor subunit 6 
Protein Synonyms/Alias
 Cleavage and polyadenylation specificity factor 68 kDa subunit; CFIm68; CPSF 68 kDa subunit; Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPBRII-4/7 
Gene Name
 CPSF6 
Gene Synonyms/Alias
 CFIM68 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
79VVYTYTGKRIALYIGubiquitination[1, 2, 3]
161PVVTPCNKQFLSQFEubiquitination[3]
185GQMSGEGKAGPPGGSubiquitination[1, 2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping. 
Sequence Annotation
 DOMAIN 81 161 RRM.
 REGION 81 161 Necessary for interaction with
 REGION 510 551 Sufficient for nuclear targeting.
 MOD_RES 404 404 Phosphothreonine.
 MOD_RES 407 407 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 551 AA 
Protein Sequence
MADGVDHIDI YADVGEEFNQ EAEYGGHDQI DLYDDVISPS ANNGDAPEDR DYMDTLPPTV 60
GDDVGKGAAP NVVYTYTGKR IALYIGNLTW WTTDEDLTEA VHSLGVNDIL EIKFFENRAN 120
GQSKGFALVG VGSEASSKKL MDLLPKRELH GQNPVVTPCN KQFLSQFEMQ SRKTTQSGQM 180
SGEGKAGPPG GSSRAAFPQG GRGRGRFPGA VPGGDRFPGP AGPGGPPPPF PGNLIKHLVK 240
GTRPLFLETR IPWHMGHSIE EIPIFGLKAG QTPPRPPLGP PGPPGPPGPP PPGQVLPPPL 300
AGPPNRGDRP PPPVLFPGQP FGQPPLGPLP PGPPPPVPGY GPPPGPPPPQ QGPPPPPGPF 360
PPRPPGPLGP PLTLAPPPHL PGPPPGAPPP APHVNPAFFP PPTNSGMPTS DSRGPPPTDP 420
YGRPPPYDRG DYGPPGREMD TARTPLSEAE FEEIMNRNRA ISSSAISRAV SDASAGDYGS 480
AIETLVTAIS LIKQSKVSAD DRCKVLISSL QDCLHGIESK SYGSGSRRER SRERDHSRSR 540
EKSRRHKSRS RDRHDDYYRE RSRERERHRD RDRDRDRERD REREYRHR 588 
Gene Ontology
 GO:0005849; C:mRNA cleavage factor complex; IDA:UniProtKB.
 GO:0042382; C:paraspeckles; IDA:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; IDA:MGI.
 GO:0003729; F:mRNA binding; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0006378; P:mRNA polyadenylation; IMP:UniProtKB.
 GO:0051262; P:protein tetramerization; IDA:UniProtKB. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
  
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS