CPLM-019569

Genbank Nucleotide ID

Transcriptional activator protein Pur-beta

Protein Synonyms/Alias

Purine-rich element-binding protein B

Homo sapiens (Human)

Position	Peptide	Type	References
60	NAKGRFLKIAEVGAG	ubiquitination	[1]
70	EVGAGGSKSRLTLSM	ubiquitination	[2]
120	GGPRRALKSEFLVRE	ubiquitination	[3]
136	RKYYLDLKENQRGRF	ubiquitination	[3]
251	FLRVSEVKPSYRNAI	ubiquitination	[2]
263	NAITVPFKAWGKFGG	ubiquitination	[2, 3]
267	VPFKAWGKFGGAFCR	ubiquitination	[2, 3]
280	CRYADEMKEIQERQR	acetylation	[4]

[1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine- rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).

DNA_BIND 28 254 By similarity.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 6 6 Phosphoserine.
MOD_RES 8 8 Phosphoserine.
MOD_RES 31 31 Phosphothreonine (By similarity).
MOD_RES 101 101 Phosphoserine.
MOD_RES 298 298 Phosphoserine.
MOD_RES 304 304 Phosphoserine.

Acetylation; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005662; C:DNA replication factor A complex; ISS:UniProtKB.
GO:0003690; F:double-stranded DNA binding; IEA:Compara.
GO:0003729; F:mRNA binding; ISS:UniProtKB.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
GO:0008134; F:transcription factor binding; ISS:UniProtKB.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
GO:0045637; P:regulation of myeloid cell differentiation; NAS:UniProtKB.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR006628; PUR_DNA_RNA-bd.