CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029117
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase 
Protein Synonyms/Alias
 cDNA FLJ60243, highly similar to Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC 2.7.11.1) 
Gene Name
 PKMYT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33AEPGFSLKRPRGLSRubiquitination[1, 2]
49LPPPPPAKGSIPISRubiquitination[1]
117GSYGEVFKVRSKEDGubiquitination[1, 3, 4, 5]
130DGRLYAVKRSMSPFRubiquitination[1]
140MSPFRGPKDRARKLAubiquitination[1]
145GPKDRARKLAEVGSHubiquitination[3]
154AEVGSHEKVGQHPCCubiquitination[1, 2]
336MMLEPDPKLRATAEAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 447 AA 
Protein Sequence
MPMPTEGTPP PLSGTPIPVP AYFRHAEPGF SLKRPRGLSR SLPPPPPAKG SIPISRLFPP 60
RTPGWHQLQP RRVSFRGEAS ETLQSPGYDP SRPESFFQQS FQRLSRLGHG SYGEVFKVRS 120
KEDGRLYAVK RSMSPFRGPK DRARKLAEVG SHEKVGQHPC CVRLEQAWEE GGILYLQTEL 180
CGPSLQQHCE AWGASLPEAQ VWGYLRDTLL ALAHLHSQGL VHLDVKPANI FLGPRGRCKL 240
GDFGLLVELG TAGAGEVQEG DPRYMAPELL QGSYGTAADV FSLGLTILEV ACNMELPHGG 300
EGWQQLRQGY LPPEFTAGLS SELRSVLVMM LEPDPKLRAT AEALLALPVL RQPRAWGVLW 360
CMAAEALSRG WALWQALLAL LCWLWHGLAH PASWLQPLGP PATPPGSPPC SLLLDSSLSS 420
NWDDDSLGPC VLKRTEGALE CSQMEGL 447 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS