CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002795
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 2',3'-cyclic-nucleotide 3'-phosphodiesterase 
Protein Synonyms/Alias
 CNP; CNPase 
Gene Name
 CNP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MNRGFSRKSHTFLPKubiquitination[1, 2]
15KSHTFLPKIFFRKMSubiquitination[3, 4, 5]
27KMSSSGAKDKPELQFmethylation[6]
29SSSGAKDKPELQFPFmethylation[6]
29SSSGAKDKPELQFPFubiquitination[1]
63RGLPGSGKSTLARVIubiquitination[1]
73LARVIVDKYRDGTKMubiquitination[1, 3, 4, 5]
79DKYRDGTKMVSADAYubiquitination[1]
87MVSADAYKITPGARGubiquitination[1, 3, 4, 5, 7, 8]
101GAFSEEYKRLDEDLAubiquitination[3, 4, 5, 7]
162RLDCAQLKEKNQWQLubiquitination[1, 8]
175QLSADDLKKLKPGLEubiquitination[3, 5]
176LSADDLKKLKPGLEKubiquitination[1]
197FGWFLTKKSSETLRKubiquitination[7]
217LEELGNHKAFKKELRubiquitination[1, 3, 4, 5, 7, 9, 10]
220LGNHKAFKKELRQFVubiquitination[1]
221GNHKAFKKELRQFVPubiquitination[1]
235PGDEPREKMDLVTYFubiquitination[1]
261TKFCDYGKAPGAEEYubiquitination[1]
275YAQQDVLKKSYSKAFubiquitination[3, 5]
276AQQDVLKKSYSKAFTubiquitination[1]
316LWPSDVDKLSPTDNLubiquitination[1, 7]
356LEILRQEKGGSRGEEubiquitination[1]
371VGELSRGKLYSLGNGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity). 
Sequence Annotation
 ACT_SITE 251 251 Proton acceptor.
 ACT_SITE 330 330 Proton donor.
 BINDING 253 253 Substrate.
 BINDING 332 332 Substrate.
 MOD_RES 110 110 Phosphotyrosine (By similarity).
 MOD_RES 373 373 Phosphotyrosine (By similarity).
 MOD_RES 418 418 Cysteine methyl ester (By similarity).
 LIPID 418 418 S-farnesyl cysteine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Hydrolase; Lipoprotein; Membrane; Methylation; Phosphoprotein; Polymorphism; Prenylation; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MNRGFSRKSH TFLPKIFFRK MSSSGAKDKP ELQFPFLQDE DTVATLLECK TLFILRGLPG 60
SGKSTLARVI VDKYRDGTKM VSADAYKITP GARGAFSEEY KRLDEDLAAY CRRRDIRILV 120
LDDTNHERER LEQLFEMADQ YQYQVVLVEP KTAWRLDCAQ LKEKNQWQLS ADDLKKLKPG 180
LEKDFLPLYF GWFLTKKSSE TLRKAGQVFL EELGNHKAFK KELRQFVPGD EPREKMDLVT 240
YFGKRPPGVL HCTTKFCDYG KAPGAEEYAQ QDVLKKSYSK AFTLTISALF VTPKTTGARV 300
ELSEQQLQLW PSDVDKLSPT DNLPRGSRAH ITLGCAADVE AVQTGLDLLE ILRQEKGGSR 360
GEEVGELSRG KLYSLGNGRW MLTLAKNMEV RAIFTGYYGK GKPVPTQGSR KGGALQSCTI 420
I 421 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:BHF-UCL.
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0005902; C:microvillus; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; IEA:Compara.
 GO:0035748; C:myelin sheath abaxonal region; IEA:Compara.
 GO:0035749; C:myelin sheath adaxonal region; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0031143; C:pseudopodium; IEA:Compara.
 GO:0004113; F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity; TAS:ProtInc.
 GO:0030551; F:cyclic nucleotide binding; IEA:Compara.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008344; P:adult locomotory behavior; IEA:Compara.
 GO:0007568; P:aging; IEA:Compara.
 GO:0007409; P:axonogenesis; IEA:Compara.
 GO:0009214; P:cyclic nucleotide catabolic process; IEA:InterPro.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara.
 GO:0046902; P:regulation of mitochondrial membrane permeability; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0009636; P:response to toxic substance; IEA:Compara.
 GO:0016070; P:RNA metabolic process; IEA:InterPro.
 GO:0007268; P:synaptic transmission; TAS:ProtInc. 
Interpro
 IPR008431; CNPase.
 IPR027417; P-loop_NTPase.
 IPR009097; RNA_ligase/cNuc_Pdiesterase. 
Pfam
 PF05881; CNPase 
SMART
  
PROSITE
  
PRINTS