CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012685
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydropyrimidinase-related protein 2 
Protein Synonyms/Alias
 DRP-2; Collapsin response mediator protein 2; CRMP-2; N2A3; Unc-33-like phosphoprotein 2; ULIP-2 
Gene Name
 DPYSL2 
Gene Synonyms/Alias
 CRMP2; ULIP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43YMEDGLIKQIGENLIubiquitination[1]
254NCPLYITKVMSKSSAubiquitination[2, 3]
258YITKVMSKSSAEVIAubiquitination[2]
269EVIAQARKKGTVVYGubiquitination[2]
270VIAQARKKGTVVYGEubiquitination[2]
293DGSHYWSKNWAKAAAacetylation[4]
293DGSHYWSKNWAKAAAubiquitination[1, 5, 6]
345TAQKAVGKDNFTLIPubiquitination[1, 2]
368RMSVIWDKAVVTGKMubiquitination[2]
374DKAVVTGKMDENQFVubiquitination[2]
390VTSTNAAKVFNLYPRubiquitination[2]
418IWDPDSVKTISAKTHubiquitination[2]
423SVKTISAKTHNSSLEubiquitination[2]
472SGRYIPRKPFPDFVYubiquitination[1, 2, 5, 6]
511CEVSVTPKTVTPASSubiquitination[2]
520VTPASSAKTSPAKQQubiquitination[2]
525SAKTSPAKQQAPPVRubiquitination[2]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (By similarity). Plays a role in neuron projection morphogenesis. 
Sequence Annotation
 MOD_RES 32 32 Phosphotyrosine; by FYN.
 MOD_RES 431 431 Phosphotyrosine (By similarity).
 MOD_RES 462 462 Phosphothreonine (By similarity).
 MOD_RES 465 465 Phosphoserine (By similarity).
 MOD_RES 499 499 Phosphotyrosine (By similarity).
 MOD_RES 509 509 Phosphothreonine.
 MOD_RES 514 514 Phosphothreonine; by GSK3-beta.
 MOD_RES 517 517 Phosphoserine.
 MOD_RES 518 518 Phosphoserine.
 MOD_RES 522 522 Phosphoserine; by DYRK2.
 MOD_RES 542 542 Phosphoserine (By similarity).
 MOD_RES 555 555 Phosphothreonine; by ROCK2 (By  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Direct protein sequencing; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 572 AA 
Protein Sequence
MSYQGKKNIP RITSDRLLIK GGKIVNDDQS FYADIYMEDG LIKQIGENLI VPGGVKTIEA 60
HSRMVIPGGI DVHTRFQMPD QGMTSADDFF QGTKAALAGG TTMIIDHVVP EPGTSLLAAF 120
DQWREWADSK SCCDYSLHVD ISEWHKGIQE EMEALVKDHG VNSFLVYMAF KDRFQLTDCQ 180
IYEVLSVIRD IGAIAQVHAE NGDIIAEEQQ RILDLGITGP EGHVLSRPEE VEAEAVNRAI 240
TIANQTNCPL YITKVMSKSS AEVIAQARKK GTVVYGEPIT ASLGTDGSHY WSKNWAKAAA 300
FVTSPPLSPD PTTPDFLNSL LSCGDLQVTG SAHCTFNTAQ KAVGKDNFTL IPEGTNGTEE 360
RMSVIWDKAV VTGKMDENQF VAVTSTNAAK VFNLYPRKGR IAVGSDADLV IWDPDSVKTI 420
SAKTHNSSLE YNIFEGMECR GSPLVVISQG KIVLEDGTLH VTEGSGRYIP RKPFPDFVYK 480
RIKARSRLAE LRGVPRGLYD GPVCEVSVTP KTVTPASSAK TSPAKQQAPP VRNLHQSGFS 540
LSGAQIDDNI PRRTTQRIVA PPGGRANITS LG 572 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0004157; F:dihydropyrimidinase activity; TAS:ProtInc.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
 GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
 GO:0021772; P:olfactory bulb development; IEA:Compara.
 GO:0014049; P:positive regulation of glutamate secretion; IEA:Compara.
 GO:0006208; P:pyrimidine nucleobase catabolic process; IEA:InterPro.
 GO:0045664; P:regulation of neuron differentiation; IEA:Compara.
 GO:0001975; P:response to amphetamine; IEA:Compara.
 GO:0042220; P:response to cocaine; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0021510; P:spinal cord development; IEA:Compara.
 GO:0048489; P:synaptic vesicle transport; IEA:Compara. 
Interpro
 IPR006680; Amidohydro_1.
 IPR011778; Hydantoinase/dihydroPyrase.
 IPR011059; Metal-dep_hydrolase_composite. 
Pfam
 PF01979; Amidohydro_1 
SMART
  
PROSITE
  
PRINTS