CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001831
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carbonic anhydrase 2 
Protein Synonyms/Alias
 Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II 
Gene Name
 CA2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18NGPEHWHKDFPIAKGubiquitination[1]
39DIDTHTAKYDPSLKPubiquitination[1]
45AKYDPSLKPLSVSYDubiquitination[1, 2]
80SQDKAVLKGGPLDGTubiquitination[1]
158SAKPGLQKVVDVLDSubiquitination[1]
224VSSEQVLKFRKLNFNubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. 
Sequence Annotation
 REGION 198 199 Substrate binding.
 ACT_SITE 64 64
 ACT_SITE 67 67
 ACT_SITE 127 127
 METAL 94 94 Zinc; catalytic.
 METAL 96 96 Zinc; catalytic.
 METAL 119 119 Zinc; catalytic.
 BINDING 62 62 Activator.
 BINDING 67 67 Activator.
 BINDING 92 92 Activator.
 MOD_RES 2 2 N-acetylserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Lyase; Metal-binding; Osteopetrosis; Polymorphism; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 260 AA 
Protein Sequence
MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL 60
NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL 120
VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP 180
RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM 240
VDNWRPAQPL KNRQIKASFK 260 
Gene Ontology
 GO:0045177; C:apical part of cell; IDA:UniProtKB.
 GO:0030424; C:axon; IEA:Compara.
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0005902; C:microvillus; IEA:Compara.
 GO:0004089; F:carbonate dehydratase activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0015701; P:bicarbonate transport; TAS:Reactome.
 GO:0015670; P:carbon dioxide transport; IEA:Compara.
 GO:0001822; P:kidney development; IEA:Compara.
 GO:0002009; P:morphogenesis of an epithelium; IEA:Compara.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0006730; P:one-carbon metabolic process; IEA:InterPro.
 GO:0045780; P:positive regulation of bone resorption; IEA:Compara.
 GO:0032849; P:positive regulation of cellular pH reduction; IEA:Compara.
 GO:0045672; P:positive regulation of osteoclast differentiation; IEA:Compara.
 GO:0043627; P:response to estrogen stimulus; IEA:Compara.
 GO:0009268; P:response to pH; IEA:Compara.
 GO:0006950; P:response to stress; IEA:Compara.
 GO:0010043; P:response to zinc ion; IEA:Compara.
 GO:0046903; P:secretion; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR001148; Carbonic_anhydrase_a.
 IPR023561; Carbonic_anhydrase_a-class.
 IPR018338; Carbonic_anhydrase_a-class_CS.
 IPR018440; Carbonic_anhydrase_CA2. 
Pfam
 PF00194; Carb_anhydrase 
SMART
 SM01057; Carb_anhydrase 
PROSITE
 PS00162; ALPHA_CA_1
 PS51144; ALPHA_CA_2 
PRINTS