CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013237
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Omega-amidase NIT2 
Protein Synonyms/Alias
 Nitrilase homolog 2 
Gene Name
 Nit2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
67KIPGESTKKLSEVAKacetylation[1]
68IPGESTKKLSEVAKEacetylation[1]
74KKLSEVAKENSIYLIacetylation[1]
123FDIDVPGKITFQESKacetylation[1]
213ASPARDEKASYVAWGacetylation[1]
249LYSDIDLKKLSEIRQacetylation[1]
262RQQIPILKQKRADLYacetylation[1]
274DLYSVESKKP*****acetylation[1]
275LYSVESKKP******acetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha- ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. 
Sequence Annotation
 DOMAIN 4 270 CN hydrolase.
 ACT_SITE 43 43 Proton acceptor (Potential).
 ACT_SITE 112 112 Potential.
 ACT_SITE 153 153 Nucleophile (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 276 AA 
Protein Sequence
MSTFRLALIQ LQVSSIKSDN ITRACSLVRE AAKQGANIVS LPECFNSPYG TNYFPEYAEK 60
IPGESTKKLS EVAKENSIYL IGGSIPEEDD GKLYNTCAVF GPDGNLLVKH RKIHLFDIDV 120
PGKITFQESK TLSPGDSFST FDTPYCRVGL GICYDMRFAE LAQIYARRGC QLLVYPGAFN 180
MTTGPAHWEL LQRARAVDNQ VYVATASPAR DEKASYVAWG HSTVVDPWGQ VLTKAGTEET 240
ILYSDIDLKK LSEIRQQIPI LKQKRADLYS VESKKP 276 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0050152; F:omega-amidase activity; IEA:EC.
 GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro. 
Interpro
 IPR003010; C-N_Hydrolase. 
Pfam
 PF00795; CN_hydrolase 
SMART
  
PROSITE
 PS50263; CN_HYDROLASE
 PS01227; UPF0012 
PRINTS