CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004793
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sodium/hydrogen exchanger 1 
Protein Synonyms/Alias
 APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; NHE-1; Solute carrier family 9 member 1 
Gene Name
 SLC9A1 
Gene Synonyms/Alias
 APNH1; NHE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
583FYHKMEMKQAIELVEubiquitination[1]
612SMQNIHPKSLPSERIubiquitination[1, 2, 3]
738DLVNEELKGKVLGLSubiquitination[1]
750GLSRDPAKVAEEDEDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. 
Sequence Annotation
 REGION 516 539 Interaction with CHP2.
 MOD_RES 599 599 Phosphoserine (By similarity).
 MOD_RES 602 602 Phosphoserine (By similarity).
 MOD_RES 605 605 Phosphoserine (By similarity).
 MOD_RES 693 693 Phosphoserine (By similarity).
 MOD_RES 697 697 Phosphoserine.
 MOD_RES 703 703 Phosphoserine.
 MOD_RES 723 723 Phosphoserine.
 MOD_RES 726 726 Phosphoserine.
 MOD_RES 729 729 Phosphoserine.
 MOD_RES 785 785 Phosphoserine.
 CARBOHYD 75 75 N-linked (GlcNAc...).  
Keyword
 3D-structure; Alternative splicing; Antiport; Calmodulin-binding; Cell membrane; Complete proteome; Endoplasmic reticulum; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 815 AA 
Protein Sequence
MVLRSGICGL SPHRIFPSLL VVVALVGLLP VLRSHGLQLS PTASTIRSSE PPRERSIGDV 60
TTAPPEVTPE SRPVNHSVTD HGMKPRKAFP VLGIDYTHVR TPFEISLWIL LACLMKIGFH 120
VIPTISSIVP ESCLLIVVGL LVGGLIKGVG ETPPFLQSDV FFLFLLPPII LDAGYFLPLR 180
QFTENLGTIL IFAVVGTLWN AFFLGGLMYA VCLVGGEQIN NIGLLDNLLF GSIISAVDPV 240
AVLAVFEEIH INELLHILVF GESLLNDAVT VVLYHLFEEF ANYEHVGIVD IFLGFLSFFV 300
VALGGVLVGV VYGVIAAFTS RFTSHIRVIE PLFVFLYSYM AYLSAELFHL SGIMALIASG 360
VVMRPYVEAN ISHKSHTTIK YFLKMWSSVS ETLIFIFLGV STVAGSHHWN WTFVISTLLF 420
CLIARVLGVL GLTWFINKFR IVKLTPKDQF IIAYGGLRGA IAFSLGYLLD KKHFPMCDLF 480
LTAIITVIFF TVFVQGMTIR PLVDLLAVKK KQETKRSINE EIHTQFLDHL LTGIEDICGH 540
YGHHHWKDKL NRFNKKYVKK CLIAGERSKE PQLIAFYHKM EMKQAIELVE SGGMGKIPSA 600
VSTVSMQNIH PKSLPSERIL PALSKDKEEE IRKILRNNLQ KTRQRLRSYN RHTLVADPYE 660
EAWNQMLLRR QKARQLEQKI NNYLTVPAHK LDSPTMSRAR IGSDPLAYEP KEDLPVITID 720
PASPQSPESV DLVNEELKGK VLGLSRDPAK VAEEDEDDDG GIMMRSKETS SPGTDDVFTP 780
APSDSPSSQR IQRCLSDPGP HPEPGEGEPF FPKGQ 815 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; TAS:UniProtKB.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0048306; F:calcium-dependent protein binding; IDA:UniProtKB.
 GO:0015385; F:sodium:hydrogen antiporter activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0055007; P:cardiac muscle cell differentiation; IEA:Compara.
 GO:0016049; P:cell growth; IEA:Compara.
 GO:0071468; P:cellular response to acidity; ISS:UniProtKB.
 GO:0030214; P:hyaluronan catabolic process; TAS:Reactome.
 GO:0070997; P:neuron death; IEA:Compara.
 GO:0045760; P:positive regulation of action potential; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0010740; P:positive regulation of intracellular protein kinase cascade; IEA:Compara.
 GO:0035794; P:positive regulation of mitochondrial membrane permeability; IEA:Compara.
 GO:0051259; P:protein oligomerization; ISS:UniProtKB.
 GO:0051453; P:regulation of intracellular pH; IDA:UniProtKB.
 GO:0051930; P:regulation of sensory perception of pain; IEA:Compara.
 GO:0002026; P:regulation of the force of heart contraction; IEA:Compara.
 GO:0001101; P:response to acid; IDA:UniProtKB.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0071436; P:sodium ion export; ISS:UniProtKB. 
Interpro
 IPR006153; Cation/H_exchanger.
 IPR018422; Cation/H_exchanger_CPA1.
 IPR001970; Na/H_exchanger_1.
 IPR004709; NaH_exchanger. 
Pfam
 PF00999; Na_H_Exchanger 
SMART
  
PROSITE
  
PRINTS
 PR01084; NAHEXCHNGR.
 PR01085; NAHEXCHNGR1.