CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001590
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase kinase kinase 6 
Protein Synonyms/Alias
 Apoptosis signal-regulating kinase 2 
Gene Name
 MAP3K6 
Gene Synonyms/Alias
 ASK2; MAPKKK6; MEKK6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
540NKVLLPAKLEVRGTDubiquitination[1]
773HIVHRDIKGDNVLINubiquitination[1, 2]
910DPFLQPGKRSRSPSSubiquitination[1, 2]
999SLLHQESKRRAMLAAubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways. 
Sequence Annotation
 DOMAIN 648 906 Protein kinase.
 NP_BIND 654 662 ATP (By similarity).
 ACT_SITE 771 771 Proton acceptor (By similarity).
 BINDING 677 677 ATP (By similarity).
 MOD_RES 806 806 Phosphothreonine (By similarity).
 MOD_RES 984 984 Phosphoserine.
 MOD_RES 1129 1129 Phosphoserine.
 MOD_RES 1149 1149 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1288 AA 
Protein Sequence
MAGPCPRSGA ERAGSCWQDP LAVALSRGRQ LAAPPGRGCA RSRPLSVVYV LTREPQPGLE 60
PREGTEAEPL PLRCLREACA QVPRPRPPPQ LRSLPFGTLE LGDTAALDAF YNADVVVLEV 120
SSSLVQPSLF YHLGVRESFS MTNNVLLCSQ ADLPDLQALR EDVFQKNSDC VGSYTLIPYV 180
VTATGRVLCG DAGLLRGLAD GLVQAGVGTE ALLTPLVGRL ARLLEATPTD SCGYFRETIR 240
RDIRQARERF SGPQLRQELA RLQRRLDSVE LLSPDIIMNL LLSYRDVQDY SAIIELVETL 300
QALPTCDVAE QHNVCFHYTF ALNRRNRPGD RAKALSVLLP LVQLEGSVAP DLYCMCGRIY 360
KDMFFSSGFQ DAGHREQAYH WYRKAFDVEP SLHSGINAAV LLIAAGQHFE DSKELRLIGM 420
KLGCLLARKG CVEKMQYYWD VGFYLGAQIL ANDPTQVVLA AEQLYKLNAP IWYLVSVMET 480
FLLYQHFRPT PEPPGGPPRR AHFWLHFLLQ SCQPFKTACA QGDQCLVLVL EMNKVLLPAK 540
LEVRGTDPVS TVTLSLLEPE TQDIPSSWTF PVASICGVSA SKRDERCCFL YALPPAQDVQ 600
LCFPSVGHCQ WFCGLIQAWV TNPDSTAPAE EAEGAGEMLE FDYEYTETGE RLVLGKGTYG 660
VVYAGRDRHT RVRIAIKEIP ERDSRFSQPL HEEIALHRRL RHKNIVRYLG SASQGGYLKI 720
FMEEVPGGSL SSLLRSVWGP LKDNESTISF YTRQILQGLG YLHDNHIVHR DIKGDNVLIN 780
TFSGLLKISD FGTSKRLAGI TPCTETFTGT LQYMAPEIID QGPRGYGKAA DIWSLGCTVI 840
EMATGRPPFH ELGSPQAAMF QVGMYKVHPP MPSSLSAEAQ AFLLRTFEPD PRLRASAQTL 900
LGDPFLQPGK RSRSPSSPRH APRPSDAPSA SPTPSANSTT QSQTFPCPQA PSQHPPSPPK 960
RCLSYGGTSQ LRVPEEPAAE EPASPEESSG LSLLHQESKR RAMLAAVLEQ ELPALAENLH 1020
QEQKQEQGAR LGRNHVEELL RCLGAHIHTP NRRQLAQELR ALQGRLRAQG LGPALLHRPL 1080
FAFPDAVKQI LRKRQIRPHW MFVLDSLLSR AVRAALGVLG PEVEKEAVSP RSEELSNEGD 1140
SQQSPGQQSP LPVEPEQGPA PLMVQLSLLR AETDRLREIL AGKEREYQAL VQRALQRLNE 1200
EARTYVLAPE PPTALSTDQG LVQWLQELNV DSGTIQMLLN HSFTLHTLLT YATRDDLIYT 1260
RIRGGMVCRI WRAILAQRAG STPVTSGP 1288 
Gene Ontology
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004709; F:MAP kinase kinase kinase activity; IDA:UniProtKB.
 GO:0007257; P:activation of JUN kinase activity; TAS:ProtInc. 
Interpro
 IPR025136; DUF4071.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR013761; SAM/pointed.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF13281; DUF4071
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS