CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030774
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal protein S6 kinase alpha-3 
Protein Synonyms/Alias
 cDNA FLJ56618, highly similar to Ribosomal protein S6 kinase alpha-3 (EC 2.7.11.1) 
Gene Name
 RPS6KA3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
246PFQGKDRKETMTMILubiquitination[1]
294PDGVEEIKRHSFFSTubiquitination[1, 2]
475KGGELLDKILRQKFFubiquitination[1, 3]
537ICDFGFAKQLRAENGubiquitination[1]
562FVAPEVLKRQGYDAAubiquitination[1, 4]
629TAKDLVSKMLHVDPHubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Ribonucleoprotein; Ribosomal protein; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 711 AA 
Protein Sequence
MDEPMGEEEI NPQTEEVSIK EIAITHHVKE GHEKADPSQF ELLKVLGQGS FGKVFLVKKI 60
SGSDARQLYA MKVLKKATLK VRDRVRTKME RDILVEVNHP FIVKLHYAFQ TEGKLYLILD 120
FLRGGDLFTR LSKEVMFTEE DVKFYLAELA LALDHLHSLG IIYRDLKPEN ILLDEEGHIK 180
LTDFGLSKES IDHEKKAYSF CGTVEYMAPE VVNRRGHTQS ADWWSFGVLM FEMLTGTLPF 240
QGKDRKETMT MILKAKLGMP QFLSPEAQSL LRMLFKRNPA NRLGAGPDGV EEIKRHSFFS 300
TIDWNKLYRR EIHPPFKPAT GRPEDTFYFD PEFTAKTPKD SPGIPPSANA HQLFRGFSFV 360
AITSDDESQA MQTVGVHSIV QLHRNSIQFT DGYEVKEDIG VGSYSVCKRC IHKATNMEFA 420
VKIIDKSKRD PTEEIEILLR YGQHPNIITL KDVYDDGKYV YVVTELMKGG ELLDKILRQK 480
FFSEREASAV LFTITKTVEY LHAQGVVHRD LKPSNILYVD ESGNPESIRI CDFGFAKQLR 540
AENGLLMTPC YTANFVAPEV LKRQGYDAAC DIWSLGVLLY TMLTGYTPFA NGPDDTPEEI 600
LARIGSGKFS LSGGYWNSVS DTAKDLVSKM LHVDPHQRLT AALVLRHPWI VHWDQLPQYQ 660
LNRQDAPHLV KGAMAATYSA LNRNQSPVLE PVGRSTLAQR RGIKKITSTA L 711 
Gene Ontology
 GO:0005840; C:ribosome; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0007243; P:intracellular protein kinase cascade; IEA:InterPro. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR011009; Kinase-like_dom.
 IPR017892; Pkinase_C.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR016239; Ribosomal_S6_kinase_II.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF00433; Pkinase_C 
SMART
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS