CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023057
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Anaphase-promoting complex subunit 5 
Protein Synonyms/Alias
 APC5; Cyclosome subunit 5 
Gene Name
 ANAPC5 
Gene Synonyms/Alias
 APC5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80ITLSKLYKLIEESCPubiquitination[1]
167QYFQNGEKKTVEDADubiquitination[2]
168YFQNGEKKTVEDADMubiquitination[2]
210CSGPLSQKQAEFFLSubiquitination[1]
224SQQASLLKNDETKALubiquitination[1, 2, 3]
229LLKNDETKALTPASLubiquitination[1, 2, 3, 4, 5]
238LTPASLQKELNNLLKubiquitination[1, 2, 4, 5]
245KELNNLLKFNPDFAEubiquitination[1, 2]
289ILTGAESKSNGEEGYubiquitination[1, 2, 3, 4, 5, 6]
366VLLEHSVKKAVHFGLubiquitination[2]
367LLEHSVKKAVHFGLPubiquitination[1]
393QQRAFAGKTANKLMDubiquitination[2]
397FAGKTANKLMDALKDubiquitination[1, 2]
403NKLMDALKDSDLLHWubiquitination[1, 2, 4, 5]
524DRAMNDGKYHLADSLubiquitination[1, 2]
547SIEGVYRKAVVLQAQubiquitination[1, 2]
562NQMSEAHKLLQKLLVubiquitination[2, 4, 5]
669RAMFLVAKCQVASAAubiquitination[1]
682AASYDQPKKAEALEAubiquitination[1]
683ASYDQPKKAEALEAAubiquitination[1, 2]
698IENLNEAKNYFAKVDubiquitination[1, 2, 3, 7]
703EAKNYFAKVDCKERIubiquitination[1]
726RLYHTLGKTQERNRCubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. 
Sequence Annotation
 REPEAT 301 334 TPR 1.
 REPEAT 522 555 TPR 2.
 REPEAT 581 614 TPR 3.
 REPEAT 678 711 TPR 4.
 MOD_RES 195 195 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Complete proteome; Mitosis; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 755 AA 
Protein Sequence
MASVHESLYF NPMMTNGVVH ANVFGIKDWV TPYKIAVLVL LNEMSRTGEG AVSLMERRRL 60
NQLLLPLLQG PDITLSKLYK LIEESCPQLA NSVQIRIKLM AEGELKDMEQ FFDDLSDSFS 120
GTEPEVHKTS VVGLFLRHMI LAYSKLSFSQ VFKLYTALQQ YFQNGEKKTV EDADMELTSR 180
DEGERKMEKE ELDVSVREEE VSCSGPLSQK QAEFFLSQQA SLLKNDETKA LTPASLQKEL 240
NNLLKFNPDF AEAHYLSYLN NLRVQDVFSS THSLLHYFDR LILTGAESKS NGEEGYGRSL 300
RYAALNLAAL HCRFGHYQQA ELALQEAIRI AQESNDHVCL QHCLSWLYVL GQKRSDSYVL 360
LEHSVKKAVH FGLPYLASLG IQSLVQQRAF AGKTANKLMD ALKDSDLLHW KHSLSELIDI 420
SIAQKTAIWR LYGRSTMALQ QAQMLLSMNS LEAVNAGVQQ NNTESFAVAL CHLAELHAEQ 480
GCFAAASEVL KHLKERFPPN SQHAQLWMLC DQKIQFDRAM NDGKYHLADS LVTGITALNS 540
IEGVYRKAVV LQAQNQMSEA HKLLQKLLVH CQKLKNTEMV ISVLLSVAEL YWRSSSPTIA 600
LPMLLQALAL SKEYRLQYLA SETVLNLAFA QLILGIPEQA LSLLHMAIEP ILADGAILDK 660
GRAMFLVAKC QVASAASYDQ PKKAEALEAA IENLNEAKNY FAKVDCKERI RDVVYFQARL 720
YHTLGKTQER NRCAMLFRQL HQELPSHGVP LINHL 755 
Gene Ontology
 GO:0005680; C:anaphase-promoting complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:ProtInc.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:ProtInc.
 GO:0000090; P:mitotic anaphase; TAS:ProtInc.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0070979; P:protein K11-linked ubiquitination; IDA:UniProtKB. 
Interpro
 IPR026000; Apc5_TPR.
 IPR011990; TPR-like_helical. 
Pfam
 PF12862; Apc5 
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS