CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022568
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2-alpha kinase 4 
Protein Synonyms/Alias
 GCN2-like protein 
Gene Name
 EIF2AK4 
Gene Synonyms/Alias
 GCN2; KIAA1338 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
610AVIKVQNKLDGCCYAubiquitination[1]
619DGCCYAVKRIPINPAubiquitination[1]
634SRQFRRIKGEVTLLSubiquitination[1]
1135RNNILNLKRYCIERVubiquitination[1]
1259NSLCRLYKFIEQKGDacetylation[2]
1489LVDHVLQKLRTKVTDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Can phosphorylate the alpha subunit of EIF2 and may mediate translational control (By similarity). 
Sequence Annotation
 DOMAIN 25 137 RWD.
 DOMAIN 296 539 Protein kinase 1.
 DOMAIN 590 1001 Protein kinase 2.
 NP_BIND 596 604 ATP (By similarity).
 REGION 1022 1493 Histidyl-tRNA synthetase-like.
 ACT_SITE 848 848 Proton acceptor (By similarity).
 BINDING 619 619 ATP (By similarity).
 MOD_RES 230 230 Phosphoserine.
 MOD_RES 667 667 Phosphothreonine.
 MOD_RES 899 899 Phosphothreonine; by autocatalysis (By
 MOD_RES 904 904 Phosphothreonine; by autocatalysis (By
 MOD_RES 1259 1259 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1649 AA 
Protein Sequence
MAGGRGAPGR GRDEPPESYP QRQDHELQAL EAIYGADFQD LRPDACGPVK EPPEINLVLY 60
PQGLTGEEVY VKVDLRVKCP PTYPDVVPEI ELKNAKGLSN ESVNLLKSRL EELAKKHCGE 120
VMIFELAYHV QSFLSEHNKP PPKSFHEEML ERRAQEEQQR LLEAKRKEEQ EQREILHEIQ 180
RRKEEIKEEK KRKEMAKQER LEIASLSNQD HTSKKDPGGH RTAAILHGGS PDFVGNGKHR 240
ANSSGRSRRE RQYSVCNSED SPGSCEILYF NMGSPDQLMV HKGKCIGSDE QLGKLVYNAL 300
ETATGGFVLL YEWVLQWQKK MGPFLTSQEK EKIDKCKKQI QGTETEFNSL VKLSHPNVVR 360
YLAMNLKEQD DSIVVDILVE HISGVSLAAH LSHSGPIPVH QLRRYTAQLL SGLDYLHSNS 420
VVHKVLSASN VLVDAEGTVK ITDYSISKRL ADICKEDVFE QTRVRFSDNA LPYKTGKKGD 480
VWRLGLLLLS LSQGQECGEY PVTIPSDLPA DFQDFLKKCV CLDDKERWSP QQLLKHSFIN 540
PQPKMPLVEQ SPEDSEGQDY VETVIPSNRL PSAAFFSETQ RQFSRYFIEF EELQLLGKGA 600
FGAVIKVQNK LDGCCYAVKR IPINPASRQF RRIKGEVTLL SRLHHENIVR YYNAWIERHE 660
RPAGPGTPPP DSGPLAKDDR AARGQPASDT DGLDSVEAAA PPPILSSSVE WSTSGERSAS 720
ARFPATGPGS SDDEDDDEDE HGGVFSQSFL PASDSESDII FDNEDENSKS QNQDEDCNEK 780
NGCHESEPSV TTEAVHYLYI QMEYCEKSTL RDTIDQGLYR DTVRLWRLFR EILDGLAYIH 840
EKGMIHRDLK PVNIFLDSDD HVKIGDFGLA TDHLAFSADS KQDDQTGDLI KSDPSGHLTG 900
MVGTALYVSP EVQGSTKSAY NQKVDLFSLG IIFFEMSYHP MVTASERIFV LNQLRDPTSP 960
KFPEDFDDGE HAKQKSVISW LLNHDPAKRP TATELLKSEL LPPPQMEESE LHEVLHHTLT 1020
NVDGKAYRTM MAQIFSQRIS PAIDYTYDSD ILKGNFSIRT AKMQQHVCET IIRIFKRHGA 1080
VQLCTPLLLP RNRQIYEHNE AALFMDHSGM LVMLPFDLRI PFARYVARNN ILNLKRYCIE 1140
RVFRPRKLDR FHPKELLECA FDIVTSTTNS FLPTAEIIYT IYEIIQEFPA LQERNYSIYL 1200
NHTMLLKAIL LHCGIPEDKL SQVYIILYDA VTEKLTRREV EAKFCNLSLS SNSLCRLYKF 1260
IEQKGDLQDL MPTINSLIKQ KTGIAQLVKY GLKDLEEVVG LLKKLGIKLQ VLINLGLVYK 1320
VQQHNGIIFQ FVAFIKRRQR AVPEILAAGG RYDLLIPQFR GPQALGPVPT AIGVSIAIDK 1380
ISAAVLNMEE SVTISSCDLL VVSVGQMSMS RAINLTQKLW TAGITAEIMY DWSQSQEELQ 1440
EYCRHHEITY VALVSDKEGS HVKVKSFEKE RQTEKRVLET ELVDHVLQKL RTKVTDERNG 1500
REASDNLAVQ NLKGSFSNAS GLFEIHGATV VPIVSVLAPE KLSASTRRRY ETQVQTRLQT 1560
SLANLHQKSS EIEILAVDLP KETILQFLSL EWDADEQAFN TTVKQLLSRL PKQRYLKLVC 1620
DEIYNIKVEK KVSVLFLYSY RDDYYRILF 1649 
Gene Ontology
 GO:0022626; C:cytosolic ribosome; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004694; F:eukaryotic translation initiation factor 2alpha kinase activity; ISS:UniProtKB.
 GO:0009267; P:cellular response to starvation; IEA:Compara.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IEA:Compara.
 GO:0017148; P:negative regulation of translation; IEA:Compara.
 GO:0006446; P:regulation of translational initiation; ISS:UniProtKB.
 GO:0043558; P:regulation of translational initiation in response to stress; IEA:Compara. 
Interpro
 IPR004154; Anticodon-bd.
 IPR026920; EIF2AK4.
 IPR024435; HisRS-related_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR006575; RWD-domain.
 IPR008271; Ser/Thr_kinase_AS.
 IPR016255; Ser/Thr_kinase_GCN2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF12745; HGTP_anticodon2
 PF00069; Pkinase
 PF05773; RWD 
SMART
 SM00591; RWD 
PROSITE
 PS50862; AA_TRNA_LIGASE_II
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50908; RWD 
PRINTS