UniProt Accession

 DHB4_DROME; Q9VXJ0 

Genbank Protein ID

 AE014298; AY051493 

Genbank Nucleotide ID

 AAF48572.1; AAK92917.1 

Protein Name

 Peroxisomal multifunctional enzyme type 2 

Protein Synonyms/Alias

 DmMFE-2; (3R)-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase 2 

Gene Name

 Mfe2 

Gene Synonyms/Alias

 CG3415 

Created Date

 July 27, 2013 

Organism

 Drosophila melanogaster (Fruit fly) 

NCBI Taxa ID

 7227 

Lysine Modification

Position	Peptide	Type	References
113	LRDRSLVKTSEQDWN	acetylation	[1]
142	QAAFPYMKKQNYGRI	acetylation	[1]
453	TFIVGAGKFGGKKDP	acetylation	[1]

Reference

 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 

Functional Description

 Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. 

Sequence Annotation

 DOMAIN 469 586 MaoC-like.
 NP_BIND 16 40 NAD (By similarity).
 NP_BIND 78 79 NAD (By similarity).
 NP_BIND 167 171 NAD (By similarity).
 NP_BIND 199 202 NAD (By similarity).
 REGION 1 309 (3R)-hydroxyacyl-CoA dehydrogenase.
 REGION 310 598 Enoyl-CoA hydratase 2.
 REGION 390 391 (3R)-3-hydroxydecanoyl-CoA binding (By
 REGION 496 501 (3R)-3-hydroxydecanoyl-CoA binding (By
 MOTIF 596 598 Microbody targeting signal (Potential).
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 24 24 NAD; via amide nitrogen (By similarity).
 BINDING 43 43 NAD (By similarity).
 BINDING 102 102 NAD; via carbonyl oxygen (By similarity).
 BINDING 154 154 Substrate (By similarity).
 BINDING 419 419 (3R)-3-hydroxydecanoyl-CoA (By
 BINDING 519 519 (3R)-3-hydroxydecanoyl-CoA; via amide
 BINDING 549 549 (3R)-3-hydroxydecanoyl-CoA; via carbonyl  

Keyword

 3D-structure; Complete proteome; Fatty acid metabolism; Lipid metabolism; Lyase; Multifunctional enzyme; NAD; Oxidoreductase; Peroxisome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 598 AA 

Protein Sequence

Gene Ontology

 GO:0005777; C:peroxisome; IC:FlyBase.
 GO:0080023; F:3R-hydroxyacyl-CoA dehydratase activity; IDA:FlyBase.
 GO:0004300; F:enoyl-CoA hydratase activity; IDA:FlyBase.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:EC.
 GO:0042803; F:protein homodimerization activity; IDA:FlyBase.
 GO:0033540; P:fatty acid beta-oxidation using acyl-CoA oxidase; IC:FlyBase. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR002539; MaoC_dom.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short
 PF01575; MaoC_dehydratas 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.