CPLM-004714

Genbank Nucleotide ID

ATP synthase-coupling factor 6, mitochondrial

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
41	KELDPIQKLFVDKIR	acetylation	[1]
41	KELDPIQKLFVDKIR	ubiquitination	[2, 3]
46	IQKLFVDKIREYKSK	acetylation	[1]
46	IQKLFVDKIREYKSK	ubiquitination	[2, 3]
79	ERELFKLKQMFGNAD	ubiquitination	[2, 3, 4]
94	MNTFPTFKFEDPKFE	acetylation	[5]
94	MNTFPTFKFEDPKFE	ubiquitination	[4]
99	TFKFEDPKFEVIEKP	acetylation	[1, 6, 7, 8]
99	TFKFEDPKFEVIEKP	ubiquitination	[2, 3]
105	PKFEVIEKPQA****	acetylation	[1, 5, 7, 8]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[6] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
[7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.

MOD_RES 41 41 N6-acetyllysine.
MOD_RES 46 46 N6-acetyllysine.
MOD_RES 99 99 N6-acetyllysine.
MOD_RES 105 105 N6-acetyllysine.

Acetylation; Alternative splicing; CF(0); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005753; C:mitochondrial proton-transporting ATP synthase complex; IDA:UniProtKB.
GO:0000276; C:mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); IEA:InterPro.
GO:0015078; F:hydrogen ion transmembrane transporter activity; IEA:InterPro.
GO:0022857; F:transmembrane transporter activity; IC:UniProtKB.
GO:0006200; P:ATP catabolic process; IDA:GOC.
GO:0042776; P:mitochondrial ATP synthesis coupled proton transport; IC:UniProtKB.
GO:0022904; P:respiratory electron transport chain; TAS:Reactome.

IPR008387; ATPase_F0-cplx_f6su_mt.
IPR016349; ATPase_F0-cplx_f6su_mt_subgr.

PF05511; ATP-synt_F6