UniProt Accession

 ASSY_ECOLI; P0A6E4; P22767; Q2M939 

Genbank Protein ID

 M35236; U18997; U00096; AP009048 

Genbank Nucleotide ID

 AAA23482.1; AAA57974.1; AAC76205.1; BAE77217.1 

Protein Name

 Argininosuccinate synthase 

Protein Synonyms/Alias

 Citrulline--aspartate ligase 

Gene Name

 argG 

Gene Synonyms/Alias

 b3172; JW3140 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
6	**MTTILKHLPVGQR	acetylation	[1]
133	WGDGSTYKGNDIERF	acetylation	[1]
189	DYKMSVEKAYSTDSN	acetylation	[1]
205	LGATHEAKDLEYLNS	acetylation	[1]
215	EYLNSSVKIVNPIMG	acetylation	[1]
224	VNPIMGVKFWDESVK	acetylation	[1]
231	KFWDESVKIPAEEVT	acetylation	[1]
252	HPVALNGKTFSDDVE	acetylation	[1]
383	VSENLTYKPERLTME	acetylation	[1]
391	PERLTMEKGDSVFSP	acetylation	[1]
418	DITDTREKLFGYAKT	acetylation	[1]
445	QVENLENKGQ*****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

  

Sequence Annotation

 NP_BIND 17 25 ATP.
 BINDING 43 43 ATP; via amide nitrogen and carbonyl
 BINDING 99 99 Citrulline.
 BINDING 129 129 ATP; via amide nitrogen.
 BINDING 131 131 Aspartate.
 BINDING 131 131 ATP.
 BINDING 135 135 Aspartate.
 BINDING 135 135 Citrulline.
 BINDING 136 136 Aspartate.
 BINDING 136 136 ATP.
 BINDING 139 139 Citrulline.
 BINDING 192 192 Citrulline.
 BINDING 194 194 ATP.
 BINDING 201 201 Citrulline.
 BINDING 203 203 Citrulline.
 BINDING 280 280 Citrulline.  

Keyword

 3D-structure; Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 447 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0006526; P:arginine biosynthetic process; IEA:HAMAP. 

Interpro

 IPR023437; Arg_succ_synth_type2_subfam.
 IPR001518; Arginosuc_synth.
 IPR018223; Arginosuc_synth_CS.
 IPR024074; AS_cat/multimer_dom_body.
 IPR024073; AS_multimer_C_tail.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00764; Arginosuc_synth 

SMART

  

PROSITE

 PS00564; ARGININOSUCCIN_SYN_1
 PS00565; ARGININOSUCCIN_SYN_2 

PRINTS