UniProt Accession

 CHSTE_HUMAN; Q8NCH0; Q6PJ31; Q6UXA0; Q96P94 

Genbank Protein ID

 AF401222; AB066595; AF282905; AK074739; BC023653; BC049214; BC053633; AY358446 

Genbank Nucleotide ID

 AAK92532.1; BAB84097.1; AAK69530.1; BAC11172.1; AAH23653.1; AAH49214.1; AAH53633.1; AAQ88811.1 

Protein Name

 Carbohydrate sulfotransferase 14 

Protein Synonyms/Alias

 Dermatan 4-sulfotransferase 1; D4ST-1; hD4ST1 

Gene Name

 CHST14 

Gene Synonyms/Alias

 D4ST1; UNQ1925/PRO4400 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
86	EGTAWRGKAPKPGGL	ubiquitination	[1]
156	FLYCYVPKVACSNWK	ubiquitination	[1]
226	LLSAYRNKFGEIREY	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. GlcUA to IdoUA. Appears to have an important role in the formation of the cerbellar neural network during postnatal brain development. 

Sequence Annotation

 NP_BIND 155 161 PAPS (By similarity).
 NP_BIND 213 221 PAPS (By similarity).
 CARBOHYD 110 110 N-linked (GlcNAc...) (Potential).
 CARBOHYD 368 368 N-linked (GlcNAc...) (Potential).  

Keyword

 Carbohydrate metabolism; Complete proteome; Disease mutation; Ehlers-Danlos syndrome; Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 376 AA 

Protein Sequence

Gene Ontology

 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0001537; F:N-acetylgalactosamine 4-O-sulfotransferase activity; IDA:UniProtKB.
 GO:0042301; F:phosphate ion binding; NAS:UniProtKB.
 GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0030204; P:chondroitin sulfate metabolic process; TAS:Reactome.
 GO:0030208; P:dermatan sulfate biosynthetic process; TAS:Reactome. 

Interpro

 IPR018011; Carb_sulfotransferase-rel.
 IPR005331; Sulfotransferase. 

Pfam

 PF03567; Sulfotransfer_2 

SMART

  

PROSITE

  

PRINTS