CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004968
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase SrmB 
Protein Synonyms/Alias
  
Gene Name
 srmB 
Gene Synonyms/Alias
 rbaB; b2576; JW2560 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
142GRLLQYIKEENFDCRacetylation[1]
244ALLVHLLKQPEATRSacetylation[1]
345RTARAGRKGTAISLVacetylation[1]
362HDHLLLGKVGRYIEEacetylation[1]
372RYIEEPIKARVIDELacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Acts as an ATP-dependent RNA helicase, able to unwind ds-RNA. 
Sequence Annotation
 DOMAIN 35 209 Helicase ATP-binding.
 DOMAIN 238 387 Helicase C-terminal.
 NP_BIND 48 55 ATP (By similarity).
 MOTIF 4 32 Q motif.
 MOTIF 157 160 DEAD box.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 444 AA 
Protein Sequence
MTVTTFSELE LDESLLEALQ DKGFTRPTAI QAAAIPPALD GRDVLGSAPT GTGKTAAYLL 60
PALQHLLDFP RKKSGPPRIL ILTPTRELAM QVSDHARELA KHTHLDIATI TGGVAYMNHA 120
EVFSENQDIV VATTGRLLQY IKEENFDCRA VETLILDEAD RMLDMGFAQD IEHIAGETRW 180
RKQTLLFSAT LEGDAIQDFA ERLLEDPVEV SANPSTRERK KIHQWYYRAD DLEHKTALLV 240
HLLKQPEATR SIVFVRKRER VHELANWLRE AGINNCYLEG EMVQGKRNEA IKRLTEGRVN 300
VLVATDVAAR GIDIPDVSHV FNFDMPRSGD TYLHRIGRTA RAGRKGTAIS LVEAHDHLLL 360
GKVGRYIEEP IKARVIDELR PKTRAPSEKQ TGKPSKKVLA KRAEKKKAKE KEKPRVKKRH 420
RDTKNIGKRR KPSGTGVPPQ TTEE 444 
Gene Ontology
 GO:0030687; C:preribosome, large subunit precursor; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:EcoCyc.
 GO:0033592; F:RNA strand annealing activity; IDA:EcoCyc.
 GO:0000027; P:ribosomal large subunit assembly; IMP:EcoCyc. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS