CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000726
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Forkhead box protein O3 
Protein Synonyms/Alias
 AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1 
Gene Name
 FOXO3 
Gene Synonyms/Alias
 FKHRL1; FOXO3A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
242IINPDGGKSGKAPRRacetylation[1]
259VSMDNSNKYTKSRGRacetylation[1]
271RGRAAKKKAALQTAPacetylation[1]
290DSPSQLSKWPGSPTSacetylation[1]
569SSSLGSAKHQQQSPVacetylation[1]
Reference
 [1] Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase.
 Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H, Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP, Gygi SP, Sinclair DA, Alt FW, Greenberg ME.
 Science. 2004 Mar 26;303(5666):2011-5. [PMID: 14976264] 
Functional Description
 Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. 
Sequence Annotation
 DNA_BIND 157 251 Fork-head.
 MOTIF 242 259 Nuclear localization signal.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 7 7 Phosphoserine.
 MOD_RES 12 12 Phosphoserine.
 MOD_RES 32 32 Phosphothreonine; by PKB/AKT1.
 MOD_RES 46 46 N6-methyllysine.
 MOD_RES 149 149 N6-methyllysine.
 MOD_RES 179 179 Phosphothreonine; by AMPK.
 MOD_RES 209 209 Phosphoserine; by STK4/MST1.
 MOD_RES 215 215 Phosphoserine; by MAPKAPK5.
 MOD_RES 230 230 N6-methyllysine.
 MOD_RES 253 253 Phosphoserine; by PKB/AKT1 and MAPKAPK5.
 MOD_RES 262 262 N6-methyllysine.
 MOD_RES 271 271 N6-methyllysine.
 MOD_RES 280 280 Phosphoserine.
 MOD_RES 284 284 Phosphoserine.
 MOD_RES 290 290 N6-methyllysine.
 MOD_RES 315 315 Phosphoserine; by SGK1.
 MOD_RES 399 399 Phosphoserine; by AMPK.
 MOD_RES 413 413 Phosphoserine; by AMPK.
 MOD_RES 419 419 N6-methyllysine.
 MOD_RES 421 421 Phosphoserine.
 MOD_RES 551 551 Phosphoserine; by MAPKAPK5.
 MOD_RES 555 555 Phosphoserine; by AMPK and MAPKAPK5.
 MOD_RES 588 588 Phosphoserine; by AMPK.
 MOD_RES 626 626 Phosphoserine; by AMPK.  
Keyword
 3D-structure; Acetylation; Activator; Apoptosis; Chromosomal rearrangement; Complete proteome; Cytoplasm; DNA-binding; Methylation; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 673 AA 
Protein Sequence
MAEAPASPAP LSPLEVELDP EFEPQSRPRS CTWPLQRPEL QASPAKPSGE TAADSMIPEE 60
EDDEDDEDGG GRAGSAMAIG GGGGSGTLGS GLLLEDSARV LAPGGQDPGS GPATAAGGLS 120
GGTQALLQPQ QPLPPPQPGA AGGSGQPRKC SSRRNAWGNL SYADLITRAI ESSPDKRLTL 180
SQIYEWMVRC VPYFKDKGDS NSSAGWKNSI RHNLSLHSRF MRVQNEGTGK SSWWIINPDG 240
GKSGKAPRRR AVSMDNSNKY TKSRGRAAKK KAALQTAPES ADDSPSQLSK WPGSPTSRSS 300
DELDAWTDFR SRTNSNASTV SGRLSPIMAS TELDEVQDDD APLSPMLYSS SASLSPSVSK 360
PCTVELPRLT DMAGTMNLND GLTENLMDDL LDNITLPPSQ PSPTGGLMQR SSSFPYTTKG 420
SGLGSPTSSF NSTVFGPSSL NSLRQSPMQT IQENKPATFS SMSHYGNQTL QDLLTSDSLS 480
HSDVMMTQSD PLMSQASTAV SAQNSRRNVM LRNDPMMSFA AQPNQGSLVN QNLLHHQHQT 540
QGALGGSRAL SNSVSNMGLS ESSSLGSAKH QQQSPVSQSM QTLSDSLSGS SLYSTSANLP 600
VMGHEKFPSD LDLDMFNGSL ECDMESIIRS ELMDADGLDF NFDSLISTQN VVGLNVGNFT 660
GAKQASSQSW VPG 673 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0008301; F:DNA binding, bending; IBA:RefGenome.
 GO:0003690; F:double-stranded DNA binding; IBA:RefGenome.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0008134; F:transcription factor binding; IBA:RefGenome.
 GO:0001547; P:antral ovarian follicle growth; IBA:RefGenome.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; IEA:Compara.
 GO:0009790; P:embryo development; IBA:RefGenome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0042593; P:glucose homeostasis; IBA:RefGenome.
 GO:0006917; P:induction of apoptosis; IBA:RefGenome.
 GO:0001544; P:initiation of primordial ovarian follicle growth; IBA:RefGenome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; IBA:RefGenome.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0001556; P:oocyte maturation; IBA:RefGenome.
 GO:0001542; P:ovulation from ovarian follicle; IBA:RefGenome.
 GO:0007389; P:pattern specification process; IBA:RefGenome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0045648; P:positive regulation of erythrocyte differentiation; IDA:MGI.
 GO:0043525; P:positive regulation of neuron apoptotic process; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; IBA:RefGenome.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0006417; P:regulation of translation; IDA:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IBA:RefGenome.
 GO:0009888; P:tissue development; IBA:RefGenome. 
Interpro
 IPR001766; TF_fork_head.
 IPR018122; TF_fork_head_CS.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00250; Fork_head 
SMART
 SM00339; FH 
PROSITE
 PS00657; FORK_HEAD_1
 PS00658; FORK_HEAD_2
 PS50039; FORK_HEAD_3 
PRINTS
 PR00053; FORKHEAD.