CPLM-030541

Genbank Nucleotide ID

Eukaryotic translation initiation factor 3 subunit F

Protein Synonyms/Alias

eIF3f; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47

Homo sapiens (Human)

Position	Peptide	Type	References
141	TLLGTVDKHSVEVTN	ubiquitination	[1]
169	AVDMEFAKNMYELHK	ubiquitination	[1]
176	KNMYELHKKVSPNEL	ubiquitination	[1]
177	NMYELHKKVSPNELI	ubiquitination	[2]
229	QNGRMSIKAYVSTLM	ubiquitination	[2]
253	MFTPLTVKYAYYDTE	acetylation	[3]
253	MFTPLTVKYAYYDTE	ubiquitination	[1, 2, 4, 5, 6, 7]
269	IGVDLIMKTCFSPNR	ubiquitination	[1]

[1] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity).

DOMAIN 104 210 MPN (By similarity).
MOD_RES 17 17 N-acetylalanine (By similarity).
MOD_RES 273 273 Phosphoserine (By similarity).

Acetylation; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
GO:0005852; C:eukaryotic translation initiation factor 3 complex; IEA:HAMAP.
GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
GO:0006446; P:regulation of translational initiation; IEA:HAMAP.

IPR027531; eIF3f.
IPR000555; JAB1_Mov34_MPN_PAD1.
IPR024969; Rpn11/EIF3F_C.

PF01398; JAB
PF13012; MitMem_reg