UniProt Accession

 MTAP_HUMAN; Q13126; I2G7M5; I2G7M6; I2G7M7; I2G7M8; I2G7M9; I2G7N0; Q5T3P3; Q9H010 

Genbank Protein ID

 U22233; L40432; L42634; L42627; L42628; L42629; L42630; L42631; L42632; L42633; HE654772; HE654773; HE654774; HE654775; HE654776; HE654777; AY712791; AL359922; CH471071; BC026106 

Genbank Nucleotide ID

 AAA81646.1; AAG38871.1; AAR24607.2; AAR24607.2; AAR24607.2; AAR24607.2; AAR24607.2; AAR24607.2; AAR24607.2; AAR24607.2; CCF77345.1; CCF77346.1; CCF77347.1; CCF77348.1; CCF77349.1; CCF77350.1; AAU04442.1; CAI16481.1; EAW58606.1; AAH26106.1 

Protein Name

 S-methyl-5'-thioadenosine phosphorylase 

Protein Synonyms/Alias

 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPase 

Gene Name

 MTAP 

Gene Synonyms/Alias

 MSAP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	ILEGRTEKYVDTPFG	ubiquitination	[1]
40	YVDTPFGKPSDALIL	ubiquitination	[1, 2, 3, 4]
49	SDALILGKIKNVDCV	ubiquitination	[1, 4]
51	ALILGKIKNVDCVLL	acetylation	[5]
71	QHTIMPSKVNYQANI	ubiquitination	[1, 3]
157	EVLIETAKKLGLRCH	ubiquitination	[4]
248	KENANKAKSLLLTTI	ubiquitination	[1, 4]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330] 

Functional Description

 Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S- adenosylmethionine. Has broad substrate specificity with 6- aminopurine nucleosides as preferred substrates. 

Sequence Annotation

 REGION 60 61 Phosphate binding.
 REGION 93 94 Phosphate binding.
 REGION 220 222 Substrate binding.
 BINDING 18 18 Phosphate.
 BINDING 196 196 Substrate; via amide nitrogen.
 BINDING 197 197 Phosphate.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Glycosyltransferase; Nucleus; Polymorphism; Purine salvage; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 283 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004645; F:phosphorylase activity; TAS:ProtInc.
 GO:0017061; F:S-methyl-5-thioadenosine phosphorylase activity; TAS:Reactome.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; TAS:Reactome.
 GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
 GO:0006595; P:polyamine metabolic process; TAS:Reactome.
 GO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW.
 GO:0033574; P:response to testosterone stimulus; IEA:Compara. 

Interpro

 IPR010044; MTAP.
 IPR000845; Nucleoside_phosphorylase_d.
 IPR001369; PNP/MTAP.
 IPR018099; Purine_phosphorylase-2_CS. 

Pfam

 PF01048; PNP_UDP_1 

SMART

  

PROSITE

 PS01240; PNP_MTAP_2 

PRINTS