CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006639
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leucine--tRNA ligase 
Protein Synonyms/Alias
 Leucyl-tRNA synthetase; LeuRS 
Gene Name
 leuS 
Gene Synonyms/Alias
 BSU30320 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
283EAVEAYIKEIQSKSDacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
  
Sequence Annotation
 MOTIF 40 51 "HIGH" region.
 MOTIF 576 580 "KMSKS" region.
 BINDING 579 579 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 804 AA 
Protein Sequence
MSFQHKEIEK KWQTYWLENK TFATLDNNEK QKFYALDMFP YPSGAGLHVG HPEGYTATDI 60
LSRMKRMQGY DVLHPMGWDA FGLPAEQYAL DTGNDPAVFT KQNIDNFRRQ IQALGFSYDW 120
DREINTTDPE YYKWTQWIFL KLYEKGLAYV DEVPVNWCPA LGTVLANEEV IDGKSERGGH 180
PVERRPMKQW MLKITAYADR LLEDLEELDW PESIKDMQRN WIGRSEGAHV HFAIDGHDDS 240
FTVFTTRPDT LFGATYTVLA PEHALVENIT TAEQKEAVEA YIKEIQSKSD LERTDLAKTK 300
TGVFTGAYAI NPVNGEKLPI WIADYVLASY GTGAVMAVPG HDERDFEFAK TFGLPVKEVV 360
KGGNVEEAAY TGDGEHVNSD FLNGLHKQEA IEKVIAWLEE TKNGEKKVTY RLRDWLFSRQ 420
RYWGEPIPVI HWEDGTSTAV PEEELPLILP KTDEIKPSGT GESPLANIKE WVEVTDPETG 480
KKGRRETNTM PQWAGSCWYF LRYIDPHNPD QLASPEKLEK WLPVDMYIGG AEHAVLHLLY 540
ARFWHKFLYD IGVVPTKEPF QKLYNQGMIL GENNEKMSKS KGNVVNPDEI VASHGADTLR 600
LYEMFMGPLD ASIAWSESGL DGARRFLDRV WRLFIEDSGE LNGKIVEGAG ETLERVYHET 660
VMKVTDHYEG LRFNTGISQL MVFINEAYKA TELPKEYMEG FVKLLSPVAP HLAEELWEKL 720
GHSGTIAYEA WPVYDETKLV DDEVEIVVQL NGKVKAKLQV PADATKEQLE QLAQADEKVK 780
EQLEGKTIRK IIAVPGKLVN IVAN 804 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004823; F:leucine-tRNA ligase activity; IEA:HAMAP.
 GO:0006429; P:leucyl-tRNA aminoacylation; IEA:HAMAP.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002300; aa-tRNA-synth_Ia.
 IPR002302; Leu-tRNA-ligase_bac/mito.
 IPR025709; Leu_tRNA-synth_edit.
 IPR015413; Methionyl/Leucyl_tRNA_Synth.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR013155; V/L/I-tRNA-synth_anticodon-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit. 
Pfam
 PF08264; Anticodon_1
 PF00133; tRNA-synt_1
 PF13603; tRNA-synt_1_2
 PF09334; tRNA-synt_1g 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00985; TRNASYNTHLEU.