CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002692
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lysine-sensitive aspartokinase 3 
Protein Synonyms/Alias
 Aspartate kinase III; AKIII; Lysine-sensitive aspartokinase III 
Gene Name
 lysC 
Gene Synonyms/Alias
 apk; b4024; JW3984 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
63EPGERFEKLDAIRNIacetylation[1]
190GFIGSENKGRTTTLGacetylation[1]
280PVFVGSSKDPRAGGTacetylation[1]
292GGTLVCNKTENPPLFacetylation[1]
404SKACGVGKEVFGVLEacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 DOMAIN 313 394 ACT.
 NP_BIND 8 11 ATP.
 NP_BIND 221 222 ATP.
 NP_BIND 257 258 ATP.
 REGION 2 245 Aspartokinase.
 REGION 198 201 Substrate binding.
 REGION 246 449 Interface.
 REGION 299 449 Required for homodimerization.
 REGION 318 321 Allosteric inhibitor binding 1.
 REGION 324 325 Allosteric inhibitor binding 1.
 REGION 338 340 Allosteric inhibitor binding 2.
 REGION 345 346 Allosteric inhibitor binding 1.
 BINDING 45 45 Substrate.
 BINDING 119 119 Substrate.
 BINDING 227 227 ATP; via amide nitrogen and carbonyl
 BINDING 232 232 ATP.  
Keyword
 3D-structure; Allosteric enzyme; Amino-acid biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Lysine biosynthesis; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 449 AA 
Protein Sequence
MSEIVVSKFG GTSVADFDAM NRSADIVLSD ANVRLVVLSA SAGITNLLVA LAEGLEPGER 60
FEKLDAIRNI QFAILERLRY PNVIREEIER LLENITVLAE AAALATSPAL TDELVSHGEL 120
MSTLLFVEIL RERDVQAQWF DVRKVMRTND RFGRAEPDIA ALAELAALQL LPRLNEGLVI 180
TQGFIGSENK GRTTTLGRGG SDYTAALLAE ALHASRVDIW TDVPGIYTTD PRVVSAAKRI 240
DEIAFAEAAE MATFGAKVLH PATLLPAVRS DIPVFVGSSK DPRAGGTLVC NKTENPPLFR 300
ALALRRNQTL LTLHSLNMLH SRGFLAEVFG ILARHNISVD LITTSEVSVA LTLDTTGSTS 360
TGDTLLTQSL LMELSALCRV EVEEGLALVA LIGNDLSKAC GVGKEVFGVL EPFNIRMICY 420
GASSHNLCFL VPGEDAEQVV QKLHSNLFE 449 
Gene Ontology
 GO:0016597; F:amino acid binding; IEA:InterPro.
 GO:0004072; F:aspartate kinase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0009090; P:homoserine biosynthetic process; IDA:EcoCyc.
 GO:0009089; P:lysine biosynthetic process via diaminopimelate; IDA:EcoCyc. 
Interpro
 IPR002912; ACT_dom.
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR005260; Asp_kin_monofn.
 IPR001341; Asp_kinase_dom.
 IPR018042; Aspartate_kinase_CS. 
Pfam
 PF00696; AA_kinase
 PF01842; ACT 
SMART
  
PROSITE
 PS51671; ACT
 PS00324; ASPARTOKINASE 
PRINTS