CPLM-007807

Genbank Nucleotide ID

Cyclin-dependent kinase inhibitor 1B

Protein Synonyms/Alias

Cyclin-dependent kinase inhibitor p27; p27Kip1

Homo sapiens (Human)

Position	Peptide	Type	References
100	RPPKGACKVPAQESQ	acetylation	[1]
165	DDSSTQNKRANRTEE	ubiquitination	[2, 3]

[1] PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1.
Pérez-Luna M, Aguasca M, Perearnau A, Serratosa J, Martínez-Balbas M, Jesús Pujol M, Bachs O.
Nucleic Acids Res. 2012 Aug;40(14):6520-33. [PMID: 22547391]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

MOTIF 153 169 Nuclear localization signal (Potential).
MOD_RES 10 10 Phosphoserine; by UHMK1.
MOD_RES 74 74 Phosphotyrosine; by SRC.
MOD_RES 88 88 Phosphotyrosine; by ABL, LYN, SRC and
MOD_RES 89 89 Phosphotyrosine.
MOD_RES 157 157 Phosphothreonine; by PKB/AKT1 and PIM1.
MOD_RES 187 187 Phosphothreonine; by PKB/AKT1 and CDK2.
MOD_RES 198 198 Phosphothreonine; by PKB/AKT1, RPS6KA1,

3D-structure; Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; Endosome; Nucleus; Phosphoprotein; Polymorphism; Protein kinase inhibitor; Proto-oncogene; Reference proteome; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; TAS:ProtInc.
GO:0005072; F:transforming growth factor beta receptor, cytoplasmic mediator activity; TAS:ProtInc.
GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:UniProtKB.
GO:0048102; P:autophagic cell death; IDA:BHF-UCL.
GO:0071236; P:cellular response to antibiotic; IEA:Compara.
GO:0071285; P:cellular response to lithium ion; IDA:MGI.
GO:0071407; P:cellular response to organic cyclic compound; IEA:Compara.
GO:0006977; P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; TAS:Reactome.
GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
GO:0000082; P:G1/S transition of mitotic cell cycle; IDA:BHF-UCL.
GO:0006917; P:induction of apoptosis; IDA:BHF-UCL.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0048839; P:inner ear development; IEA:Compara.
GO:0071850; P:mitotic cell cycle arrest; IDA:UniProtKB.
GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
GO:0030308; P:negative regulation of cell growth; IDA:BHF-UCL.
GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
GO:0051271; P:negative regulation of cellular component movement; IEA:Compara.
GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IEA:Compara.
GO:0060770; P:negative regulation of epithelial cell proliferation involved in prostate gland development; IEA:Compara.
GO:0045930; P:negative regulation of mitotic cell cycle; IDA:UniProtKB.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
GO:0031116; P:positive regulation of microtubule polymerization; IEA:Compara.
GO:0045732; P:positive regulation of protein catabolic process; IDA:MGI.
GO:0006813; P:potassium ion transport; IEA:Compara.
GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; TAS:ProtInc.
GO:0007605; P:sensory perception of sound; IEA:Compara.