UniProt Accession

 B3KSP0_HUMAN; B3KSP0 

Genbank Protein ID

 AL132868; AK094040; CH471131 

Genbank Nucleotide ID

 BAG52802.1; EAW82547.1 

Protein Name

 Negative elongation factor A 

Protein Synonyms/Alias

 Wolf-Hirschhorn syndrome candidate 2, isoform CRA_c; cDNA FLJ36721 fis, clone UTERU2011287, highly similar to Negative elongation factor A 

Gene Name

 WHSC2 

Gene Synonyms/Alias

 NELFA; hCG_18088 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
26	GPLTPPVKHFQLKRK	ubiquitination	[1]
55	TETAQQLKRSAGVPF	ubiquitination	[1]
236	PTLPAQFKQRAPMYN	ubiquitination	[2]
311	FAAQEMFKTANKVTR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Elongation factor; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 393 AA 

Protein Sequence

Gene Ontology

 GO:0032021; C:NELF complex; IEA:InterPro.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
 GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:Compara. 

Interpro

 IPR026305; NELF-A. 

Pfam

  

SMART

  

PROSITE

  

PRINTS