UniProt Accession

 F2Z3V0_MOUSE; F2Z3V0 

Genbank Protein ID

 AL591952 

Genbank Nucleotide ID

  

Protein Name

 NADH-cytochrome b5 reductase 3 

Protein Synonyms/Alias

  

Gene Name

 Cyb5r3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
19	TLENPDIKYPLRLID	acetylation	[1, 2, 3, 4, 5, 6, 7]
19	TLENPDIKYPLRLID	ubiquitination	[8]
27	YPLRLIDKEVISPDT	acetylation	[3, 6]
27	YPLRLIDKEVISPDT	succinylation	[6]
27	YPLRLIDKEVISPDT	ubiquitination	[8]
80	PVSSDDDKGFVDLVV	acetylation	[7]
92	LVVKVYFKDTHPKFP	acetylation	[7]
92	LVVKVYFKDTHPKFP	ubiquitination	[8]
97	YFKDTHPKFPAGGKM	acetylation	[2, 6, 7]
97	YFKDTHPKFPAGGKM	ubiquitination	[8]
103	PKFPAGGKMSQYLEN	acetylation	[3, 6]
103	PKFPAGGKMSQYLEN	succinylation	[6]
103	PKFPAGGKMSQYLEN	ubiquitination	[8]
112	SQYLENMKIGDTIEF	ubiquitination	[8]
131	GLLVYQGKGKFAIRA	ubiquitination	[8]

Reference

 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 144 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:Compara.
 GO:0005811; C:lipid particle; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0016208; F:AMP binding; IEA:Compara.
 GO:0071949; F:FAD binding; IEA:Compara.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0016491; F:oxidoreductase activity; IEA:InterPro. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6 

SMART

  

PROSITE

 PS51384; FAD_FR 

PRINTS

 PR00406; CYTB5RDTASE.