UniProt Accession

 CLPP_HUMAN; Q16740; B2R4W5 

Genbank Protein ID

 Z50853; AK311973; BC002956 

Genbank Nucleotide ID

 CAA90705.1; BAG34912.1; AAH02956.1 

Protein Name

 Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial 

Protein Synonyms/Alias

 Endopeptidase Clp 

Gene Name

 CLPP 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
202	AEEIMKLKKQLYNIY	ubiquitination	[1]
203	EEIMKLKKQLYNIYA	ubiquitination	[1]
211	QLYNIYAKHTKQSLQ	acetylation	[2]
211	QLYNIYAKHTKQSLQ	ubiquitination	[1]
214	NIYAKHTKQSLQVIE	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. 

Sequence Annotation

 ACT_SITE 153 153 Probable.
 ACT_SITE 178 178 Probable.
 MOD_RES 211 211 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Hydrolase; Mitochondrion; Nucleotide-binding; Protease; Reference proteome; Serine protease; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 277 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008233; F:peptidase activity; TAS:ProtInc.
 GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR001907; ClpP.
 IPR023562; ClpP/TepA.
 IPR018215; ClpP_AS. 

Pfam

 PF00574; CLP_protease 

SMART

  

PROSITE

 PS00382; CLP_PROTEASE_HIS
 PS00381; CLP_PROTEASE_SER 

PRINTS

 PR00127; CLPPROTEASEP.