CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015317
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fidgetin-like protein 1 
Protein Synonyms/Alias
  
Gene Name
 FIGNL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30SGICTGPKADAYRAQubiquitination[1]
124KMMQAGKKFKDSLLEubiquitination[1]
126MQAGKKFKDSLLEPAubiquitination[1]
141LASVVIHKEATVFDLubiquitination[2]
183FPESNRLKLLQNAQPubiquitination[1]
225TPLFGNVKKENHSSAubiquitination[1]
226PLFGNVKKENHSSAKubiquitination[1]
258ACENPQRKSFYGSGTubiquitination[1, 3, 4]
276LSNPILNKACSKTEDubiquitination[1]
309LWVDQQKKYHQPQRAubiquitination[1]
339RSRGILGKFVPPIPKacetylation[5]
359QNGGMQCKPYGAGPTubiquitination[2]
658VRPSVSPKDLELYENubiquitination[2]
668ELYENWNKTFGCGK*ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 May regulate osteoblast proliferation and differentiation (By similarity). 
Sequence Annotation
 NP_BIND 444 449 ATP.
 BINDING 404 404 ATP; via amide nitrogen and carbonyl
 MOD_RES 259 259 Phosphoserine.
 MOD_RES 339 339 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Hydrolase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 674 AA 
Protein Sequence
MQTSSSRSVH LSEWQKNYFA ITSGICTGPK ADAYRAQILR IQYAWANSEI SQVCATKLFK 60
KYAEKYSAII DSDNVESGLN NYAENILTLA GSQQTDSDKW QSGLSINNVF KMSSVQKMMQ 120
AGKKFKDSLL EPALASVVIH KEATVFDLPK FSVCGSSQES DSLPNSAHDR DRTQDFPESN 180
RLKLLQNAQP PMVTNTARTC PTFSAPVGES ATAKFHVTPL FGNVKKENHS SAKENIGLNV 240
FLSNQSCFPA ACENPQRKSF YGSGTIDALS NPILNKACSK TEDNGPKEDS SLPTFKTAKE 300
QLWVDQQKKY HQPQRASGSS YGGVKKSLGA SRSRGILGKF VPPIPKQDGG EQNGGMQCKP 360
YGAGPTEPAH PVDERLKNLE PKMIELIMNE IMDHGPPVNW EDIAGVEFAK ATIKEIVVWP 420
MLRPDIFTGL RGPPKGILLF GPPGTGKTLI GKCIASQSGA TFFSISASSL TSKWVGEGEK 480
MVRALFAVAR CQQPAVIFID EIDSLLSQRG DGEHESSRRI KTEFLVQLDG ATTSSEDRIL 540
VVGATNRPQE IDEAARRRLV KRLYIPLPEA SARKQIVINL MSKEQCCLSE EEIEQIVQQS 600
DAFSGADMTQ LCREASLGPI RSLQTADIAT ITPDQVRPIA YIDFENAFRT VRPSVSPKDL 660
ELYENWNKTF GCGK 674 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016787; F:hydrolase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0046034; P:ATP metabolic process; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0001649; P:osteoblast differentiation; ISS:UniProtKB.
 GO:0033687; P:osteoblast proliferation; ISS:UniProtKB.
 GO:0051726; P:regulation of cell cycle; ISS:UniProtKB. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase.
 IPR015415; Vps4_C. 
Pfam
 PF00004; AAA
 PF09336; Vps4_C 
SMART
 SM00382; AAA 
PROSITE
 PS00674; AAA 
PRINTS