CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020403
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras-related protein Rab-34 
Protein Synonyms/Alias
 Ras-related protein Rab-39; Ras-related protein Rah 
Gene Name
 RAB34 
Gene Synonyms/Alias
 RAB39; RAH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
168LFLVGSKKDLSTPAQubiquitination[1, 2]
227NVLAELEKSGARRIGubiquitination[3]
252NLYLTASKKKPTCCPubiquitination[1, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 
Functional Description
 Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region (By similarity). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. 
Sequence Annotation
 NP_BIND 59 66 GTP (By similarity).
 NP_BIND 107 111 GTP (By similarity).
 NP_BIND 166 169 GTP (By similarity).
 MOTIF 81 89 Effector region (By similarity).
 MOD_RES 241 241 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 LIPID 257 257 S-geranylgeranyl cysteine (Potential).
 LIPID 258 258 S-geranylgeranyl cysteine (Potential).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Golgi apparatus; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Prenylation; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 259 AA 
Protein Sequence
MNILAPVRRD RVLAELPQCL RKEAALHGHK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD 60
LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGIP FSLQLWDTAG QERFKCIAST 120
YYRGAQAIII VFNLNDVASL EHTKQWLADA LKENDPSSVL LFLVGSKKDL STPAQYALME 180
KDALQVAQEM KAEYWAVSSL TGENVREFFF RVAALTFEAN VLAELEKSGA RRIGDVVRIN 240
SDDSNLYLTA SKKKPTCCP 259 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0045335; C:phagocytic vesicle; IDA:UniProtKB.
 GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0090385; P:phagosome-lysosome fusion; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 
Pfam
 PF00071; Ras 
SMART
 SM00175; RAB 
PROSITE
 PS51419; RAB 
PRINTS
 PR00449; RASTRNSFRMNG.