CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006089
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TATA-binding protein-associated factor MOT1 
Protein Synonyms/Alias
 TBP-associated factor MOT1; Modifier of transcription 1 
Gene Name
 MOT1 
Gene Synonyms/Alias
 YPL082C; LPF4C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
101PLDNAQVKLEHEMKIsumoylation[1]
109LEHEMKIKLEEATQNsumoylation[1]
141WKLNEILKSGKVLLAubiquitination[2]
159NDYNVLGKADDNIRKacetylation[3]
229ITESSVSKTLLNGKNacetylation[3]
519SIMDLLAKLCDHQEVubiquitination[2]
Reference
 [1] Quality control of a transcriptional regulator by SUMO-targeted degradation.
 Wang Z, Prelich G.
 Mol Cell Biol. 2009 Apr;29(7):1694-706. [PMID: 19139279]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box via its C-terminal ATPase activity. Both transcription activation and repression require its ATPase activity. 
Sequence Annotation
 REPEAT 289 326 HEAT 1.
 REPEAT 445 482 HEAT 2.
 REPEAT 541 578 HEAT 3.
 REPEAT 1108 1145 HEAT 4.
 REPEAT 1188 1225 HEAT 5.
 DOMAIN 1284 1457 Helicase ATP-binding.
 REPEAT 1495 1537 HEAT 6.
 DOMAIN 1639 1787 Helicase C-terminal.
 NP_BIND 1297 1304 ATP (Potential).
 MOTIF 195 211 Nuclear localization signal (Potential).
 MOTIF 1408 1411 DEGH box.
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 677 677 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; Mitochondrion; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1867 AA 
Protein Sequence
MTSRVSRLDR QVILIETGST QVVRNMAADQ MGDLAKQHPE DILSLLSRVY PFLLVKKWET 60
RVTAARAVGG IVAHAPSWDP NESDLVGGTN EGSPLDNAQV KLEHEMKIKL EEATQNNQLN 120
LLQEDHHLSS LSDWKLNEIL KSGKVLLASS MNDYNVLGKA DDNIRKQAKT DDIKQETSML 180
NASDKANENK SNANKKSARM LAMARRKKKM SAKNTPKHPV DITESSVSKT LLNGKNMTNS 240
AASLATSPTS NQLNPKLEIT EQADESKLMI ESTVRPLLEQ HEIVAGLVWQ FQGIYELLLD 300
NLMSENWEIR HGAALGLREL VKKHAYGVSR VKGNTREENN LRNSRSLEDL ASRLLTVFAL 360
DRFGDYVYDT VVAPVRESVA QTLAALLIHL DSTLSIKIFN CLEQLVLQDP LQTGLPNKIW 420
EATHGGLLGI RYFVSIKTNF LFAHGLLENV VRIVLYGLNQ SDDDVQSVAA SILTPITSEF 480
VKLNNSTIEI LVTTIWSLLA RLDDDISSSV GSIMDLLAKL CDHQEVLDIL KNKALEHPSE 540
WSFKSLVPKL YPFLRHSISS VRRAVLNLLI AFLSIKDDST KNWLNGKVFR LVFQNILLEQ 600
NPELLQLSFD VYVALLEHYK VKHTEKTLDH VFSKHLQPIL HLLNTPVGEK GKNYAMESQY 660
ILKPSQHYQL HPEKKRSISE TTTDSDIPIP KNNEHINIDA PMIAGDITLL GLDVILNTRI 720
MGAKAFALTL SMFQDSTLQS FFTNVLVRCL ELPFSTPRML AGIIVSQFCS SWLQKHPEGE 780
KLPSFVSEIF SPVMNKQLLN RDEFPVFREL VPSLKALRTQ CQSLLATFVD VGMLPQYKLP 840
NVAIVVQGET EAGPHAFGVE TAEKVYGEYY DKMFKSMNNS YKLLAKKPLE DSKHRVLMAI 900
NSAKESAKLR TGSILANYAS SILLFDGLPL KLNPIIRSLM DSVKEERNEK LQTMAGESVV 960
HLIQQLLENN KVNVSGKIVK NLCGFLCVDT SEVPDFSVNA EYKEKILTLI KESNSIAAQD 1020
DINLAKMSEE AQLKRKGGLI TLKILFEVLG PSILQKLPQL RSILFDSLSD HENEEASKVD 1080
NEQGQKIVDS FGVLRALFPF MSDSLRSSEV FTRFPVLLTF LRSNLSVFRY SAARTFADLA 1140
KISSVEVMAY TIREILPLMN SAGSLSDRQG STELIYHLSL SMETDVLPYV IFLIVPLLGR 1200
MSDSNEDVRN LATTTFASII KLVPLEAGIA DPKGLPEELV ASRERERDFI QQMMDPSKAK 1260
PFKLPIAIKA TLRKYQQDGV NWLAFLNKYH LHGILCDDMG LGKTLQTICI IASDQYLRKE 1320
DYEKTRSVES RALPSLIICP PSLTGHWENE FDQYAPFLKV VVYAGGPTVR LTLRPQLSDA 1380
DIIVTSYDVA RNDLAVLNKT EYNYCVLDEG HIIKNSQSKL AKAVKEITAN HRLILTGTPI 1440
QNNVLELWSL FDFLMPGFLG TEKMFQERFA KPIAASRNSK TSSKEQEAGV LALEALHKQV 1500
LPFMLRRLKE DVLSDLPPKI IQDYYCELGD LQKQLYMDFT KKQKNVVEKD IENSEIADGK 1560
QHIFQALQYM RKLCNHPALV LSPNHPQLAQ VQDYLKQTGL DLHDIINAPK LSALRTLLFE 1620
CGIGEEDIDK KASQDQNFPI QNVISQHRAL IFCQLKDMLD MVENDLFKKY MPSVTYMRLD 1680
GSIDPRDRQK VVRKFNEDPS IDCLLLTTKV GGLGLNLTGA DTVIFVEHDW NPMNDLQAMD 1740
RAHRIGQKKV VNVYRIITKG TLEEKIMGLQ KFKMNIASTV VNQQNSGLAS MDTHQLLDLF 1800
DPDNVTSQDN EEKNNGDSQA AKGMEDIANE TGLTGKAKEA LGELKELWDP SQYEEEYNLD 1860
TFIKTLR 1867 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0000228; C:nuclear chromosome; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0003677; F:DNA binding; IDA:SGD.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0045898; P:regulation of RNA polymerase II transcriptional preinitiation complex assembly; IMP:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD.
 GO:0042790; P:transcription of nuclear large rRNA transcript from RNA polymerase I promoter; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR022707; DUF3535.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF12054; DUF3535
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS50077; HEAT_REPEAT
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS