| Tag | Content |
|---|
| CPLM ID | CPLM-007532 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | RNA end formation protein 2 |
Protein Synonyms/Alias | |
Gene Name | REF2 |
Gene Synonyms/Alias | YDR195W; YD9346.06 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 288 | SFSKYLNKDDADMTK | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Negative regulator of poly(A) synthesis. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. REF2 is required for 3'-end formation of snoRNAs. |
Sequence Annotation | |
Keyword | Complete proteome; mRNA processing; Nucleus; Reference proteome; RNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 533 AA |
Protein Sequence | MSAPVPQLVN ISHALQASTI QQIRLDMVDF NKDCKLSSIQ LARIDKYIDS LQAALNQFTK 60
DNLHIERKEK NVTEADIQLY SGLKSMYLDY LNQLIKLKHE KQHHSTPPIA NDVSLDFFVN 120
QLPKFSPEER KNYIDNLILN KNSHNRLSKM DGLVDAVINL CVLDTSVAEN VRSYMKLLDT 180
LGFQKGSNST GTKANLKKKL ASSKAKIKDS EKEKEKEKDK SKVKMKTKLK PSPLLNNDDK 240
NSSPSPTAST SSMKKLKSGL FNKNEAKSTE SLPTSSKKKL SFSKYLNKDD ADMTKLGTKR 300
SIDVDFKVNP EASTVASNII SSSTSGSSTT TVATPASSEE PLKKKTKISV QDSNVQSILR 360
NGKPKKARIS SIKFLDDSQL IKVYGDDLPN QGLQVSPTQL KKILKPFKEG EPKEIILFED 420
MSIKLKPLDL MFLKNTNSDD YMDISETKGG PIHCETRTPL IYRKNFNHFN PDLNKRPPRE 480
PIEFDLNGNT NSTPTIAKAF GKNSLLLRKD RGGLPYKHVP IVKRNKYPPR PVH 533 |
Gene Ontology | GO:0005829; C:cytosol; IDA:SGD. GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IDA:SGD. GO:0003682; F:chromatin binding; IDA:SGD. GO:0003723; F:RNA binding; IDA:SGD. GO:0031124; P:mRNA 3'-end processing; IMP:SGD. GO:1900364; P:negative regulation of mRNA polyadenylation; IMP:SGD. GO:0031126; P:snoRNA 3'-end processing; IMP:SGD. GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |