UniProt Accession

 RNPS1_HUMAN; Q15287; A8K1P0; B4DDU8; B4DZU7; B7ZA17; O75308; Q32P25; Q8WY42; Q9NYG3 

Genbank Protein ID

 L37368; AF015608; AF274003; AK289955; AK293343; AK303100; AK316132; AC009065; CH471112; BC001659; BC001838; BC108316; AF247662 

Genbank Nucleotide ID

 AAA92859.1; AAC39791.1; AAL56665.1; BAF82644.1; BAG56859.1; BAG64209.1; BAH14503.1; EAW85516.1; AAH01659.1; AAH01838.1; AAI08317.1; AAF72519.1 

Protein Name

 RNA-binding protein with serine-rich domain 1 

Protein Synonyms/Alias

 SR-related protein LDC2 

Gene Name

 RNPS1 

Gene Synonyms/Alias

 LDC2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
188	FSTYGKIKMIDMPVE	ubiquitination	[1]
203	RMHPHLSKGYAYVEF	ubiquitination	[2]
218	ENPDEAEKALKHMDG	acetylation	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Part of pre- and post- splicing multiprotein mRNP complexes. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Participates in mRNA 3'-end cleavage. Involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions. 

Sequence Annotation

 DOMAIN 161 240 RRM.
 REGION 1 220 Necessary for interaction with the
 REGION 1 161 Necessary for interaction with SRP54,
 REGION 69 121 Necessary for interactions with UPF2 and
 REGION 156 242 Necessary for interaction with PNN and
 REGION 238 305 Necessary for interaction with TRA2B,
 MOD_RES 53 53 Phosphoserine; by CK2.
 MOD_RES 155 155 Phosphoserine.
 MOD_RES 157 157 Phosphoserine.
 MOD_RES 161 161 Phosphothreonine.
 MOD_RES 218 218 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; mRNA processing; mRNA splicing; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 305 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003730; F:mRNA 3'-UTR binding; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:UniProtKB.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS