CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018583
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Twinfilin-1 
Protein Synonyms/Alias
 Protein A6 
Gene Name
 Twf1 
Gene Synonyms/Alias
 Ptk9 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
136VSLHGYKKYLLSQSSacetylation[1]
192DAFQALEKLSKKQLNubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. 
Sequence Annotation
 DOMAIN 2 139 ADF-H 1.
 DOMAIN 175 313 ADF-H 2.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 143 143 Phosphoserine (By similarity).
 MOD_RES 309 309 Phosphotyrosine.
 MOD_RES 349 349 Phosphothreonine.  
Keyword
 3D-structure; Acetylation; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVVG SCSPPSDSWE QDYDSFVLPL 60
LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE 120
VFGTVKEDVS LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVSVDT KHQTLQGVAF 180
PISRDAFQAL EKLSKKQLNY VQLEIDIKNE TIILANTENT ELRDLPKRIP KDSARYHFFL 240
YKHSHEGDYL ESVVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMD VIRKIEIDNG 300
DELTADFLYD EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAEAEATTD 350 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:UniProtKB.
 GO:0005911; C:cell-cell junction; IDA:BHF-UCL.
 GO:0030175; C:filopodium; IDA:BHF-UCL.
 GO:0030016; C:myofibril; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0003785; F:actin monomer binding; IDA:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:Compara.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:BHF-UCL.
 GO:0004713; F:protein tyrosine kinase activity; IDA:UniProtKB.
 GO:0051016; P:barbed-end actin filament capping; IDA:BHF-UCL.
 GO:0043538; P:regulation of actin phosphorylation; IEA:Compara.
 GO:0042989; P:sequestering of actin monomers; IDA:BHF-UCL. 
Interpro
 IPR002108; Actin-bd_cofilin/tropomyosin. 
Pfam
 PF00241; Cofilin_ADF 
SMART
 SM00102; ADF 
PROSITE
 PS51263; ADF_H 
PRINTS