CPLM-024353

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-024353

UniProt Accession

PLCH2_MOUSE; A2AP18; A6PWW5; Q3LUA7; Q4QSC7; Q80WP6; Q8BJV1; Q8BWU4; Q8BXN5; Q8CFR1

Genbank Protein ID

DQ176851; AL831788; BX004788; BX004788; AL831788; AL831788; BX004788; BX004788; AL831788; AK044619; AK049970; AK078731; AY966876; BC040465; BC052329

Genbank Nucleotide ID

ABA12210.1; CAM16344.2; CAM16344.2; CAM28037.2; CAM28037.2; CAO78007.1; CAO78007.1; CAO78129.1; CAO78129.1; BAC32005.1; BAC34011.1; BAC37371.1; AAY33831.1; AAH40465.1; AAH52329.1

Protein Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2

Protein Synonyms/Alias

Phosphoinositide phospholipase C-eta-2; Phosphoinositide phospholipase C-like 4; PLC-L4; Phospholipase C-like protein 4; Phospholipase C-eta-2; PLC-eta2

Gene Name

Plch2

Gene Synonyms/Alias

Plcl4

Created Date

July 27, 2013

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Lysine Modification

Position	Peptide	Type	References
479	DVIETINKYAFIKNE	ubiquitination	[1]

Reference

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain.

Sequence Annotation

DOMAIN 121 229 PH.
DOMAIN 243 278 EF-hand 1.
DOMAIN 279 315 EF-hand 2.
DOMAIN 400 545 PI-PLC X-box.
DOMAIN 707 821 PI-PLC Y-box.
DOMAIN 826 933 C2.
REGION 75 229 Necessary for plasma membrane
ACT_SITE 415 415 By similarity.
ACT_SITE 459 459 By similarity.
METAL 416 416 Calcium 1; catalytic (By similarity).
METAL 445 445 Calcium 1; catalytic (By similarity).
METAL 447 447 Calcium 1; catalytic (By similarity).
METAL 494 494 Calcium 1; catalytic (By similarity).
METAL 865 865 Calcium 2; via carbonyl oxygen (By
METAL 867 867 Calcium 2 (By similarity).
METAL 891 891 Calcium 2 (By similarity).
METAL 920 920 Calcium 3 (By similarity).
METAL 921 921 Calcium 3; via carbonyl oxygen (By
METAL 922 922 Calcium 3 (By similarity).
BINDING 543 543 Substrate (By similarity).
BINDING 545 545 Substrate (By similarity).
BINDING 734 734 Substrate (By similarity).
BINDING 761 761 Substrate (By similarity).
MOD_RES 676 676 Phosphoserine.
MOD_RES 686 686 Phosphoserine.

Keyword

Alternative splicing; Calcium; Cell membrane; Complete proteome; Cytoplasm; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Transducer.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1501 AA

Protein Sequence

MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL 60
SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM 120
SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE 180
IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR 240
QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL 300
GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE 360
QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV 420
GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY 480
AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI 540
LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK 600
ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS 660
FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH 720
DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW 780
NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ 840
LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE 900
TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV 960
AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP 1020
SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRDTR LFPLQRPISP 1080
LCSLEPIAEE PALGPGLPLQ AAAPTGPSQE GSQCPVGLGA KVTSSQQTSL GAFGTLQLRI 1140
GGGRENEEPP LRPHNGGISS GPREGTSGRQ TDSKSRSRVP GHLPVVRRAK SEGQVLSELS 1200
PTPAVYSDAT GTDRLWQRLE PGSHRDSVSS SSSMSSNDTV IDLSLPSLGL CRSRESIPGV 1260
SLGRLTSRPC LASAARPDLP PVTKSKSNPN LRVAGGLPTA PDELQPRPLA PRLTGHHPRP 1320
PWHHLTLVGL RDCPVSAKSK SLGDLTADDF APSFQGSTSS LSCGLGSLGV AHQVLEPGIR 1380
RDALTEQLRW LTGFQQAGDI TSPTSLGPAG DGSVGGPSFL RRSSSRSQSR VRAIASRARQ 1440
AQERQQRLRG QDSRGPPEEE RGTPEGACSV GHEGCVDVPM PAKGAPEQVC GAADGQLLLR 1500
L 1501

Gene Ontology

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; IDA:MGI.
GO:0005509; F:calcium ion binding; IEA:InterPro.
GO:0004435; F:phosphatidylinositol phospholipase C activity; IEA:EC.
GO:0004629; F:phospholipase C activity; IDA:MGI.
GO:0005543; F:phospholipid binding; IEA:InterPro.
GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
GO:0035556; P:intracellular signal transduction; IEA:InterPro.
GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
GO:0046488; P:phosphatidylinositol metabolic process; IDA:MGI.

Interpro

IPR000008; C2_Ca-dep.
IPR008973; C2_Ca/lipid-bd_dom_CaLB.
IPR018029; C2_membr_targeting.
IPR011992; EF-hand-like_dom.
IPR018247; EF_Hand_1_Ca_BS.
IPR002048; EF_hand_dom.
IPR011993; PH_like_dom.
IPR001192; Pinositol_PLipase_C.
IPR017946; PLC-like_Pdiesterase_TIM-brl.
IPR001849; Pleckstrin_homology.
IPR015359; PLipase_C_EF-hand-like.
IPR000909; PLipase_C_PInositol-sp_X_dom.
IPR001711; PLipase_C_Pinositol-sp_Y.

Pfam

PF00168; C2
PF13499; EF_hand_5
PF09279; efhand_like
PF00388; PI-PLC-X
PF00387; PI-PLC-Y

SMART

SM00239; C2
SM00054; EFh
SM00233; PH
SM00148; PLCXc
SM00149; PLCYc

PROSITE

PS50004; C2
PS00018; EF_HAND_1
PS50222; EF_HAND_2
PS50003; PH_DOMAIN
PS50007; PIPLC_X_DOMAIN
PS50008; PIPLC_Y_DOMAIN

PRINTS

PR00390; PHPHLIPASEC.