CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017440
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Macoilin 
Protein Synonyms/Alias
 Transmembrane protein 57 
Gene Name
 TMEM57 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
177FKSYVSYKMRLRKQKubiquitination[1, 2]
188RKQKEVQKENEFYMQacetylation[3]
210PEQQMLQKQEKEAEEubiquitination[1]
242GGIPANKKLSTTLPEubiquitination[2, 4, 5]
265EKDKDAKKHNLGINNubiquitination[6]
282ILQPVDSKIQEIEYMubiquitination[6]
296MENHINSKRLNNDLVubiquitination[4, 5, 7]
311GSTENLLKEDSCTASubiquitination[6]
320DSCTASSKNYKNASGubiquitination[6]
323TASSKNYKNASGVVNubiquitination[6, 7]
365SKSPSTHKDLMENCIubiquitination[4, 5, 6]
438ENELLQNKLHNAVQMubiquitination[4, 5]
579LSAETRIKLDLFSALubiquitination[4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
  
Sequence Annotation
 MOD_RES 332 332 Phosphoserine.
 MOD_RES 631 631 Phosphoserine.
 MOD_RES 634 634 Phosphoserine.
 CARBOHYD 324 324 N-linked (GlcNAc...) (Potential).
 CARBOHYD 340 340 N-linked (GlcNAc...) (Potential).
 CARBOHYD 452 452 N-linked (GlcNAc...) (Potential).
 CARBOHYD 655 655 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell projection; Complete proteome; Glycoprotein; Membrane; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 664 AA 
Protein Sequence
MKRRNADCSK LRRPLKRNRI TEGIYGSTFL YLKFLVVWAL VLLADFVLEF RFEYLWPFWL 60
FIRSVYDSFR YQGLAFSVFF VCVAFTSNII CLLFIPIQWL FFAASTYVWV QYVWHTERGV 120
CLPTVSLWIL FVYIEAAIRF KDLKNFHVDL CRPFAAHCIG YPVVTLGFGF KSYVSYKMRL 180
RKQKEVQKEN EFYMQLLQQA LPPEQQMLQK QEKEAEEAAK GLPDMDSSIL IHHNGGIPAN 240
KKLSTTLPEI EYREKGKEKD KDAKKHNLGI NNNNILQPVD SKIQEIEYME NHINSKRLNN 300
DLVGSTENLL KEDSCTASSK NYKNASGVVN SSPRSHSATN GSIPSSSSKN EKKQKCTSKS 360
PSTHKDLMEN CIPNNQLSKP DALVRLEQDI KKLKADLQAS RQVEQELRSQ ISSLSSTERG 420
IRSEMGQLRQ ENELLQNKLH NAVQMKQKDK QNISQLEKKL KAEQEARSFV EKQLMEEKKR 480
KKLEEATAAR AVAFAAASRG ECTETLRNRI RELEAEGKKL TMDMKVKEDQ IRELELKVQE 540
LRKYKENEKD TEVLMSALSA MQDKTQHLEN SLSAETRIKL DLFSALGDAK RQLEIAQGQI 600
LQKDQEIKDL KQKIAEVMAV MPSITYSAAT SPLSPVSPHY SSKFVETSPS GLDPNASVYQ 660
PLKK 664 
Gene Ontology
 GO:0030424; C:axon; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0044306; C:neuron projection terminus; ISS:BHF-UCL.
 GO:0031965; C:nuclear membrane; ISS:BHF-UCL.
 GO:0045202; C:synapse; ISS:BHF-UCL.
 GO:0007420; P:brain development; IEA:Compara. 
Interpro
 IPR019130; Macoilin. 
Pfam
 PF09726; Macoilin 
SMART
  
PROSITE
  
PRINTS