CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012312
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UDP-glucose 4-epimerase 
Protein Synonyms/Alias
 Galactowaldenase; UDP-galactose 4-epimerase 
Gene Name
 GALE 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
100AVGESVQKPLDYYRVacetylation[1]
100AVGESVQKPLDYYRVubiquitination[2, 3, 4, 5, 6]
120IQLLEIMKAHGVKNLubiquitination[3]
161TNPYGKSKFFIEEMIubiquitination[3, 5]
176RDLCQADKTWNAVLLubiquitination[5, 7]
249IHVVDLAKGHIAALRubiquitination[3, 8]
286QMVQAMEKASGKKIPubiquitination[3]
295SGKKIPYKVVARREGubiquitination[5]
338EDLWRWQKQNPSGFGubiquitination[3, 4, 5, 8, 9]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose and the epimerization of UDP-N-acetylglucosamine to UDP-N- acetylgalactosamine. 
Sequence Annotation
 NP_BIND 4 35 NAD.
 ACT_SITE 157 157 Proton acceptor.
 BINDING 132 132 Substrate.  
Keyword
 3D-structure; Carbohydrate metabolism; Complete proteome; Disease mutation; Galactose metabolism; Isomerase; NAD; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 348 AA 
Protein Sequence
MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV 60
EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK 120
AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA 180
VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD 240
YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR 300
EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA 348 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0003978; F:UDP-glucose 4-epimerase activity; IDA:UniProtKB.
 GO:0044237; P:cellular metabolic process; IEA:InterPro.
 GO:0019388; P:galactose catabolic process; IDA:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR025308; Epimerase_C.
 IPR001509; Epimerase_deHydtase.
 IPR005886; GalE.
 IPR016040; NAD(P)-bd_dom.
 IPR008089; Nuc_sugar_epim. 
Pfam
 PF01370; Epimerase
 PF13950; Epimerase_Csub 
SMART
  
PROSITE
  
PRINTS
 PR01713; NUCEPIMERASE.