UniProt Accession

 R27AA_ARATH; P59271; O80715; P59263; Q29PZ3; Q38875; Q9LDJ2; Q9LYW1; Q9M0W3; Q9M1P9; Q9S7X3 

Genbank Protein ID

 AC005292; AC007945; CP002684; BT024763; AY085389 

Genbank Nucleotide ID

 AAF87001.1; AAF79581.1; AEE30384.1; ABD59101.1; AAM62617.1 

Protein Name

 Ubiquitin-40S ribosomal protein S27a-1 

Protein Synonyms/Alias

 Ubiquitin; 40S ribosomal protein S27a-1 

Gene Name

 RPS27AA 

Gene Synonyms/Alias

 UBQ16; At1g23410; F26F24.28; F28C11.5 

Created Date

 July 27, 2013 

Organism

 Arabidopsis thaliana (Mouse-ear cress) 

NCBI Taxa ID

 3702 

Lysine Modification

Position	Peptide	Type	References
11	FVKTLTGKTITLEVE	ubiquitination	[1]
29	TIDNVKAKIQDKEGI	ubiquitination	[1]
33	VKAKIQDKEGIPPDQ	ubiquitination	[1]
48	QRLIFAGKQLEDGRT	ubiquitination	[1]
63	LADYNIQKESTLHLV	ubiquitination	[1]

Reference

 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265] 

Functional Description

 Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA- damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). 

Sequence Annotation

 DOMAIN 1 76 Ubiquitin-like.
 ZN_FING 121 144 C4-type.
 CROSSLNK 11 11 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 29 29 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 33 33 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 48 48 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 63 63 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 76 76 Glycyl lysine isopeptide (Gly-Lys)  

Keyword

 Complete proteome; Cytoplasm; Isopeptide bond; Metal-binding; Nucleus; Reference proteome; Ribonucleoprotein; Ribosomal protein; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 156 AA 

Protein Sequence

Gene Ontology

 GO:0022627; C:cytosolic small ribosomal subunit; IDA:TAIR.
 GO:0005794; C:Golgi apparatus; IDA:TAIR.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:TAIR.
 GO:0009506; C:plasmodesma; IDA:TAIR.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0006412; P:translation; IEA:InterPro. 

Interpro

 IPR002906; Ribosomal_S27a.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019956; Ubiquitin_subgr.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF01599; Ribosomal_S27
 PF00240; ubiquitin 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 

PRINTS

 PR00348; UBIQUITIN.