CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010188
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC 
Protein Synonyms/Alias
 23S rRNA(m5U747)-methyltransferase 
Gene Name
 rlmC 
Gene Synonyms/Alias
 rumB; ybjF; b0859; JW0843 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
69VVSGSVEKPLLGMLHacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA. 
Sequence Annotation
 ACT_SITE 334 334 Nucleophile (By similarity).
 METAL 3 3 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 11 11 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 14 14 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 87 87 Iron-sulfur (4Fe-4S) (By similarity).
 BINDING 212 212 S-adenosyl-L-methionine (By similarity).
 BINDING 241 241 S-adenosyl-L-methionine; via carbonyl
 BINDING 262 262 S-adenosyl-L-methionine (By similarity).
 BINDING 307 307 S-adenosyl-L-methionine (By similarity).  
Keyword
 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding; Methyltransferase; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 375 AA 
Protein Sequence
MQCALYDAGR CRSCQWIMQP IPEQLSAKTA DLKNLLADFP VEEWCAPVSG PEQGFRNKAK 60
MVVSGSVEKP LLGMLHRDGT PEDLCDCPLY PASFAPVFAA LKPFIARAGL TPYNVARKRG 120
ELKYILLTES QSDGGMMLRF VLRSDTKLAQ LRKALPWLHE QLPQLKVITV NIQPVHMAIM 180
EGETEIYLTE QQALAERFND VPLWIRPQSF FQTNPAVASQ LYATARDWVR QLPVKHMWDL 240
FCGVGGFGLH CATPDMQLTG IEIASEAIAC AKQSAAELGL TRLQFQALDS TQFATAQGDV 300
PELVLVNPPR RGIGKPLCDY LSTMAPRFII YSSCNAQTMA KDIRELPGFR IERVQLFDMF 360
PHTAHYEVLT LLVKQ 375 
Gene Ontology
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0005506; F:iron ion binding; IEA:HAMAP.
 GO:0016436; F:rRNA (uridine) methyltransferase activity; IMP:EcoCyc.
 GO:0070041; F:rRNA (uridine-C5-)-methyltransferase activity; IEA:HAMAP.
 GO:0070475; P:rRNA base methylation; IMP:EcoCyc. 
Interpro
 IPR011825; 23SrRNA_MeTrfase_RlmC.
 IPR010280; U5_MeTrfase. 
Pfam
 PF05958; tRNA_U5-meth_tr 
SMART
  
PROSITE
 PS01230; TRMA_1
 PS01231; TRMA_2 
PRINTS