UniProt Accession

 SIR5_HUMAN; Q9NXA8; B4DFM4; B4DYJ5; F5H5Z9; Q5T294; Q5T295; Q9Y6E6 

Genbank Protein ID

 AF083110; AK000355; AK294162; AK302467; AM393414; AL441883; AL441883; CH471087; BC000126 

Genbank Nucleotide ID

 AAD40853.1; BAA91107.1; BAG57485.1; BAG63757.1; CAL38292.1; CAI19837.1; CAI19838.1; EAW55332.1; AAH00126.1 

Protein Name

 NAD-dependent protein deacylase sirtuin-5, mitochondrial 

Protein Synonyms/Alias

 Regulatory protein SIR2 homolog 5; SIR2-like protein 5 

Gene Name

 SIRT5 

Gene Synonyms/Alias

 SIR2L5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
203	DASIPVEKLPRCEEA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. 

Sequence Annotation

 DOMAIN 41 309 Deacetylase sirtuin-type.
 NP_BIND 58 77 NAD.
 NP_BIND 140 143 NAD (By similarity).
 NP_BIND 249 251 NAD.
 NP_BIND 275 277 NAD.
 ACT_SITE 158 158 Proton acceptor.
 METAL 166 166 Zinc.
 METAL 169 169 Zinc.
 METAL 207 207 Zinc.
 METAL 212 212 Zinc.
 BINDING 102 102 Substrate.
 BINDING 105 105 Substrate.
 BINDING 293 293 NAD; via amide nitrogen.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Mitochondrion; NAD; Polymorphism; Reference proteome; Transit peptide; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 310 AA 

Protein Sequence

Gene Ontology

 GO:0005758; C:mitochondrial intermembrane space; IDA:UniProtKB.
 GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
 GO:0003950; F:NAD+ ADP-ribosyltransferase activity; TAS:ProtInc.
 GO:0070403; F:NAD+ binding; IDA:UniProtKB.
 GO:0036054; F:protein-malonyllysine demalonylase activity; IDA:UniProtKB.
 GO:0036055; F:protein-succinyllysine desuccinylase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0006342; P:chromatin silencing; TAS:ProtInc.
 GO:0006471; P:protein ADP-ribosylation; TAS:ProtInc.
 GO:0006476; P:protein deacetylation; IDA:UniProtKB. 

Interpro

 IPR003000; Sirtuin.
 IPR026591; Sirtuin_cat_small_dom.
 IPR027546; Sirtuin_ClassIII.
 IPR026590; Ssirtuin_cat_dom. 

Pfam

 PF02146; SIR2 

SMART

  

PROSITE

 PS50305; SIRTUIN 

PRINTS