UniProt Accession

 VRK3_HUMAN; Q8IV63; A6NEG5; A8KA53; Q502Y2; Q9P2V8 

Genbank Protein ID

 AB031052; AF514788; AK292918; AK303010; AC011452; BC023556; BC095449 

Genbank Nucleotide ID

 BAA90769.1; AAP47180.1; BAF85607.1; BAG64141.1; AAH23556.1; AAH95449.1 

Protein Name

 Inactive serine/threonine-protein kinase VRK3 

Protein Synonyms/Alias

 Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3 

Gene Name

 VRK3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
161	TGTVLTDKSGRQWKL	ubiquitination	[1]
169	SGRQWKLKSFQTRDN	ubiquitination	[1]
197	TCDSGPQKQKFSLKL	ubiquitination	[1]
199	DSGPQKQKFSLKLDA	ubiquitination	[1]
229	AKPLQVNKWKKLYST	ubiquitination	[2, 3]
275	SALDVSPKHVLSERS	ubiquitination	[1, 2, 3]
338	FRYCPSGKHVAYVEG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus (By similarity). 

Sequence Annotation

 DOMAIN 166 457 Protein kinase.
 MOTIF 49 64 Nuclear localization signal.
 MOD_RES 55 55 Phosphoserine.
 MOD_RES 59 59 Phosphoserine.
 MOD_RES 82 82 Phosphoserine.
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 90 90 Phosphoserine.
 MOD_RES 115 115 Phosphoserine.
 MOD_RES 122 122 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 474 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004672; F:protein kinase activity; IEA:InterPro. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS