UniProt Accession

 CBX1_HUMAN; P83916; P23197 

Genbank Protein ID

 U35451; BC002609; BC021302 

Genbank Nucleotide ID

 AAB81548.1; AAH02609.1; AAH21302.1 

Protein Name

 Chromobox protein homolog 1 

Protein Synonyms/Alias

 HP1Hsbeta; Heterochromatin protein 1 homolog beta; HP1 beta; Heterochromatin protein p25; M31; Modifier 1 protein; p25beta 

Gene Name

 CBX1 

Gene Synonyms/Alias

 CBX 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	EEEYVVEKVLDRRVV	ubiquitination	[1, 2]
41	GKVEYLLKWKGFSDE	ubiquitination	[1, 3]
139	LMFLMKWKNSDEADL	ubiquitination	[1, 2, 3, 4]
150	EADLVPAKEANVKCP	ubiquitination	[1, 2, 5]
180	YPSEDDDKKDDKN**	ubiquitination	[4, 6, 7]
181	PSEDDDKKDDKN***	ubiquitination	[4, 7]
184	DDDKKDDKN******	ubiquitination	[2, 7]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. 

Sequence Annotation

 DOMAIN 21 79 Chromo 1.
 DOMAIN 117 175 Chromo 2; shadow subtype.
 BINDING 21 21 Histone H3K9me2 (By similarity).
 BINDING 23 23 Histone H3A7 (By similarity).
 BINDING 40 40 Histone H3A7 (By similarity).
 BINDING 42 42 Histone H3A7 (By similarity).
 BINDING 42 42 Histone H3K9me2 (By similarity).
 BINDING 45 45 Histone H3K9me2 (By similarity).
 BINDING 58 58 Histone H3A7 (By similarity).
 BINDING 60 60 Histone H3A7 (By similarity).
 BINDING 125 125 PxVxL motif (By similarity).
 BINDING 126 126 PxVxL motif (By similarity).
 BINDING 135 135 PxVxL motif (By similarity).
 BINDING 146 146 PxVxL motif (By similarity).
 BINDING 163 163 PxVxL motif (By similarity).
 BINDING 167 167 PxVxL motif (By similarity).
 BINDING 168 168 PxVxL motif (By similarity).
 BINDING 170 170 PxVxL motif (By similarity).
 MOD_RES 89 89 Phosphoserine.
 MOD_RES 91 91 Phosphoserine.
 MOD_RES 175 175 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 185 AA 

Protein Sequence

Gene Ontology

 GO:0005721; C:centromeric heterochromatin; IEA:Compara.
 GO:0010369; C:chromocenter; IEA:Compara.
 GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
 GO:0001939; C:female pronucleus; IEA:Compara.
 GO:0001940; C:male pronucleus; IEA:Compara.
 GO:0005720; C:nuclear heterochromatin; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:ProtInc.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; TAS:ProtInc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara. 

Interpro

 IPR017984; Chromo_dom_subgr.
 IPR023780; Chromo_domain.
 IPR000953; Chromo_domain/shadow.
 IPR008251; Chromo_shadow_dom.
 IPR016197; Chromodomain-like.
 IPR023779; Chromodomain_CS. 

Pfam

 PF00385; Chromo
 PF01393; Chromo_shadow 

SMART

 SM00298; CHROMO
 SM00300; ChSh 

PROSITE

 PS00598; CHROMO_1
 PS50013; CHROMO_2 

PRINTS

 PR00504; CHROMODOMAIN.